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FBXW7 Antibody (MA5-50723) in IHC (P)
Immunohistochemistry of FBXW7 in paraffin-embedded human stomach carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FBXW7 recombinant monoclonal antibody (Product # MA5-50723) with a dilution of 1:500 for 1 ... View More
Product Details
MA5-50723
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-51
Immunogen
Synthetic peptide aa 84-327/707.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093009
Product Specific Information
Positive control: Human bladder carcinoma tissue, human colon carcinoma tissue, human stomach carcinoma tissue.
Subcellular Location: Chromosome, Cytoplasm, Nucleus.
Target Information
F-Box and WD Repeat Domain Containing 7 (FBXW7) is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW7 was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: archipelago; Archipelago homolog; DKFZp686F23254; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; F-box/WD repeat-containing protein 7; FLJ11071; FLJ16457; hAgo; hCdc4; homolog of C elegans sel-10; SEL-10
Gene Aliases: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; FBXW7; hAgo; hCdc4; SEL-10; SEL10
UniProt ID: (Human) Q969H0
Entrez Gene ID: (Human) 55294
protein polyubiquitination
vasculature development
sister chromatid cohesion
negative regulation of triglyceride biosynthetic process
regulation of lipid storage
viral process
protein ubiquitination
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
positive regulation of protein ubiquitination
negative regulation of DNA endoreduplication
regulation of protein localization
positive regulation of epidermal growth factor-activated receptor activity
negative regulation of Notch signaling pathway
protein stabilization
positive regulation of ubiquitin-protein transferase activity
lipid homeostasis
positive regulation of ERK1 and ERK2 cascade
positive regulation of proteasomal protein catabolic process
regulation of cell cycle G1/S phase transition
regulation of mitophagy
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
positive regulation of protein targeting to mitochondrion
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of hepatocyte proliferation
negative regulation of SREBP signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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