Proteintech

FECH Polyclonal Antibody

FECH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FECH Antibody (14466-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human heart tissue slide using 14466-1-AP ( FECH Antibody) at dilution of 1:200 (under 40x lens).

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Product Details

14466-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FECH Fusion Protein Ag5814 (60-423 aa encoded by BC039841)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VQPQKRKPK TGILMLNMGG PETLGDVHDF LLRLFLDRDL MTLPIQNKLA PFIAKRRTPK IQEQYRRIGG GSPIKIWTSK QGEGMVKLLD ELSPNTAPHK YYIGFRYVHP LTEEAIEEME RDGLERAIAF TQYPQYSCST TGSSLNAIYR YYNQVGRKPT MKWSTIDRWP THHLLIQCFA DHILKELDHF PLEKRSEVVI LFSAHSLPMS VVNRGDPYPQ EVSATVQKVM ERLEYCNPYR LVWQSKVGPM PWLGPQTDES IKGLCERGRK NILLVPIAFT SDHIETLYEL DIEYSQVLAK ECGVENIRRA ESLNGNPLFS KALADLVHSH IQSNELCSKQ LTLSCPLCVN PVCRETKSFF TSQQL (60-423 aa encoded by B C039841)

Target Information

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cell senescence-associated porphyrin metabolism affects the efficacy of aminolevulinic acid-photodynamic diagnosis in bladder cancer.

Photodiagnosis and photodynamic therapy

Lai HW,Yamamoto S,Fukuhara H,Ogura SI,Inoue K

Thu Jun 01 00:00:00 EDT 2023

Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Brain communications

Maio N,Saneto RP,Steet R,Sotero de Menezes MA,Skinner C,Rouault TA

Sat Sep 16 00:00:00 EDT 2023

Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.

Frontiers in medicine

Mischkulnig M,Roetzer-Pejrimovsky T,Lötsch-Gojo D,Kastner N,Bruckner K,Prihoda R,Lang A,Martinez-Moreno M,Furtner J,Berghoff A,Woehrer A,Berger W,Widhalm G,Kiesel B

Sat Jul 16 00:00:00 EDT 2022

NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins.

Cancer discovery

Santana-Codina N,Del Rey MQ,Kapner KS,Zhang H,Gikandi A,Malcolm C,Poupault C,Kuljanin M,John KM,Biancur DE,Chen B,Das NK,Lowder KE,Hennessey CJ,Huang W,Yang A,Shah YM,Nowak JA,Aguirre AJ,Mancias JD

Fri Sep 02 00:00:00 EDT 2022

ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.

Nature cancer

Zhang T,Sun L,Hao Y,Suo C,Shen S,Wei H,Ma W,Zhang P,Wang T,Gu X,Li ST,Chen Z,Yan R,Zhang Y,Cai Y,Zhou R,Jia W,Huang F,Gao P,Zhang H

Sat Jan 01 00:00:00 EST 2022

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Cell reports

Acoba MG,Alpergin ESS,Renuse S,Fernández-Del-Río L,Lu YW,Khalimonchuk O,Clarke CF,Pandey A,Wolfgang MJ,Claypool SM

Tue Mar 16 00:00:00 EDT 2021

Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells.

Nature metabolism

Schwartz AJ,Goyert JW,Solanki S,Kerk SA,Chen B,Castillo C,Hsu PP,Do BT,Singhal R,Dame MK,Lee HJ,Spence JR,Lakhal-Littleton S,Vander Heiden MG,Lyssiotis CA,Xue X,Shah YM

Thu Jul 01 00:00:00 EDT 2021

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Nature

Wang Y,Yen FS,Zhu XG,Timson RC,Weber R,Xing C,Liu Y,Allwein B,Luo H,Yeh HW,Heissel S,Unlu G,Gamazon ER,Kharas MG,Hite R,Birsoy K

Mon Nov 01 00:00:00 EDT 2021

Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.

Cell death & disease

Jing J,Ding N,Wang D,Ge X,Ma J,Ma R,Huang X,Jueraitetibaike K,Liang K,Wang S,Cao S,Zhao AZ,Yao B

Fri Aug 14 00:00:00 EDT 2020

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

Molecular cell

Weber RA,Yen FS,Nicholson SPV,Alwaseem H,Bayraktar EC,Alam M,Timson RC,La K,Abu-Remaileh M,Molina H,Birsoy K

Thu Feb 06 00:00:00 EST 2020

Nitric oxide orchestrates metabolic rewiring in M1 macrophages by targeting aconitase 2 and pyruvate dehydrogenase.

Nature communications

Palmieri EM,Gonzalez-Cotto M,Baseler WA,Davies LC,Ghesquière B,Maio N,Rice CM,Rouault TA,Cassel T,Higashi RM,Lane AN,Fan TW,Wink DA,McVicar DW

Tue Feb 04 00:00:00 EST 2020

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Haematologica

Maio N,Kim KS,Holmes-Hampton G,Singh A,Rouault TA

Sun Sep 01 00:00:00 EDT 2019

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Cell metabolism

Maio N,Kim KS,Singh A,Rouault TA

Tue Apr 04 00:00:00 EDT 2017

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Cell metabolism

Maio N,Kim KS,Singh A,Rouault TA

Tue Apr 04 00:00:00 EDT 2017

Involvement of protoporphyrin IX accumulation in the pathogenesis of isoniazid/rifampicin-induced liver injury: the prevention of curcumin.

Xenobiotica; the fate of foreign compounds in biological systems

He L,Guo Y,Deng Y,Li C,Zuo C,Peng W

Wed Feb 01 00:00:00 EST 2017

Involvement of protoporphyrin IX accumulation in the pathogenesis of isoniazid/rifampicin-induced liver injury: the prevention of curcumin.

Xenobiotica; the fate of foreign compounds in biological systems

He L,Guo Y,Deng Y,Li C,Zuo C,Peng W

Wed Feb 01 00:00:00 EST 2017

Heme levels are increased in human failing hearts.

Journal of the American College of Cardiology

Khechaduri A,Bayeva M,Chang HC,Ardehali H

Tue May 07 00:00:00 EDT 2013

Heme levels are increased in human failing hearts.

Journal of the American College of Cardiology

Khechaduri A,Bayeva M,Chang HC,Ardehali H

Tue May 07 00:00:00 EDT 2013

MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Journal of the American Heart Association

Qiao A,Khechaduri A,Kannan Mutharasan R,Wu R,Nagpal V,Ardehali H

Mon Apr 22 00:00:00 EDT 2013

MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Journal of the American Heart Association

Qiao A,Khechaduri A,Kannan Mutharasan R,Wu R,Nagpal V,Ardehali H

Mon Apr 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Ferrochelatase, mitochondrial; Heme synthase; heme synthetase; Protoheme ferro-lyase; protoporphyria

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Gene Aliases: AI894116; EPP; FCE; fch; Fcl; FECH

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UniProt ID: (Human) P22830, (Mouse) P22315

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Entrez Gene ID: (Human) 2235, (Rat) 361338, (Mouse) 14151

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Function(s)

ferrochelatase activity protein binding ferrous iron binding 2 iron, 2 sulfur cluster binding iron ion binding heme binding iron-responsive element binding tetrapyrrole binding lyase activity metal ion binding iron-sulfur cluster binding lyase

Process(es)

generation of precursor metabolites and energy heme biosynthetic process response to light stimulus response to lead ion response to insecticide response to drug response to ethanol protoporphyrinogen IX metabolic process response to arsenic-containing substance response to methylmercury response to platinum ion cellular response to dexamethasone stimulus porphyrin-containing compound biosynthetic process cholesterol metabolic process detection of UV response to metal ion regulation of gene expression regulation of eIF2 alpha phosphorylation by heme erythrocyte differentiation very-low-density lipoprotein particle assembly heme metabolic process regulation of hemoglobin biosynthetic process iron ion homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FECH Polyclonal Antibody

FECH Antibody (14466-1-AP) in IHC (P)

Product Details

14466-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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