Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
FECH Antibody (PA5-29733) in WB
Western Blot analysis of FECH was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FECH Polyclonal Antibody (Product # PA5-29733) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Product Details
PA5-29733
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 129 and 370 of Human Ferrochelatase
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547207
Product Specific Information
Recommended positive controls: HeLa, HepG2.
Predicted reactivity: Mouse (92%), Rat (93%), Xenopus laevis (87%), Pig (89%), Chicken (85%), Chimpanzee (100%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Ferrochelatase, mitochondrial; Heme synthase; heme synthetase; Protoheme ferro-lyase; protoporphyria
Gene Aliases: EPP; FCE; FECH
UniProt ID: (Human) P22830
Entrez Gene ID: (Human) 2235
generation of precursor metabolites and energy
heme biosynthetic process
response to light stimulus
response to lead ion
response to insecticide
response to drug
response to ethanol
protoporphyrinogen IX metabolic process
response to arsenic-containing substance
response to methylmercury
response to platinum ion
cellular response to dexamethasone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support