Proteintech

FEN1 Polyclonal Antibody

FEN1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

FEN1 Antibody (14768-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 14768-1-AP (FEN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FEN1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14768-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FEN1 Fusion Protein Ag6552 (1-380 aa encoded by BC000323)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGIQGLAKL IADVAPSAIR ENDIKSYFGR KVAIDASMSI YQFLIAVRQG GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K (1-380 aa encoded by BC000323)

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CJ: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane.

Journal of medicinal chemistry

Fan R,Deng A,Qi B,Zhang S,Sang R,Luo L,Gou J,Liu Y,Lin R,Zhao M,Liu Y,Yang L,Cheng M,Wei G

Thu Jan 12 00:00:00 EST 2023

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Nature communications

Jhanji M,Rao CN,Massey JC,Hope MC,Zhou X,Keene CD,Ma T,Wyatt MD,Stewart JA,Sajish M

Fri Jun 10 00:00:00 EDT 2022

HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.

Cell reports

Fu S,Phan AT,Mao D,Wang X,Gao G,Goff SP,Zhu Y

Tue May 24 00:00:00 EDT 2022

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Biology

Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

Fri Feb 11 00:00:00 EST 2022

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

American journal of physiology. Cell physiology

Shi R,Wang Y,Gao Y,Xu X,Mao S,Xiao Y,Song S,Wang L,Tian B,Zhao Y,Hua Y,Xu H

Thu Oct 01 00:00:00 EDT 2020

Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma.

Disease markers

Zhang Y,Liu X,Liu L,Chen J,Hu Q,Shen S,Zhou Y,Chen S,Xue C,Cui G,Yu Z

Fri Apr 02 00:00:00 EDT 2021

Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma.

Disease markers

Zhang Y,Liu X,Liu L,Chen J,Hu Q,Shen S,Zhou Y,Chen S,Xue C,Cui G,Yu Z

Fri Apr 02 00:00:00 EDT 2021

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Journal of molecular cell biology

Xu X,Shi R,Zheng L,Guo Z,Wang L,Zhou M,Zhao Y,Tian B,Truong K,Chen Y,Shen B,Hua Y,Xu H

Mon Oct 01 00:00:00 EDT 2018

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Journal of molecular cell biology

Xu X,Shi R,Zheng L,Guo Z,Wang L,Zhou M,Zhao Y,Tian B,Truong K,Chen Y,Shen B,Hua Y,Xu H

Mon Oct 01 00:00:00 EDT 2018

Synergistic antitumor effect of combined paclitaxel with FEN1 inhibitor in cervical cancer cells.

DNA repair

He L,Yang H,Zhou S,Zhu H,Mao H,Ma Z,Wu T,Kumar AK,Kathera C,Janardhan A,Pan F,Hu Z,Yang Y,Luo L,Guo Z

Thu Mar 01 00:00:00 EST 2018

Synergistic antitumor effect of combined paclitaxel with FEN1 inhibitor in cervical cancer cells.

DNA repair

He L,Yang H,Zhou S,Zhu H,Mao H,Ma Z,Wu T,Kumar AK,Kathera C,Janardhan A,Pan F,Hu Z,Yang Y,Luo L,Guo Z

Thu Mar 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: DNase IV; FEN-1; FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}; FEN1; fen1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap endonuclease 1; flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap structure-specific endonuclease 1; flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; hFEN-1; Maturation factor 1; maturation factor-1; MF1

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Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2

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UniProt ID: (Human) P39748, (Rat) Q5XIP6

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Entrez Gene ID: (Human) 2237, (Mouse) 14156, (Rat) 84490

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Function(s)

magnesium ion binding DNA binding damaged DNA binding double-stranded DNA binding endonuclease activity RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding double-stranded DNA exodeoxyribonuclease activity 5'-3' exonuclease activity 5'-flap endonuclease activity catalytic activity nuclease activity hydrolase activity hydrolase activity, acting on ester bonds manganese ion binding phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity metal ion binding flap endonuclease activity exodeoxyribonuclease

Process(es)

telomere maintenance via recombination double-strand break repair via homologous recombination DNA replication DNA repair base-excision repair double-strand break repair memory UV protection DNA replication, removal of RNA primer RNA phosphodiester bond hydrolysis, endonucleolytic DNA metabolic process cellular response to DNA damage stimulus nucleic acid phosphodiester bond hydrolysis DNA catabolic process, endonucleolytic

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FEN1 Polyclonal Antibody

FEN1 Antibody (14768-1-AP) in ICC/IF

Product Details

14768-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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