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FGF17 Antibody (500-P152BT-25UG) in ELISA
Direct ELISA: To detect Human FGF-17 by direct ELISA (using 100 µL/well) a concentration of approximately 1.0 µg/mL of FGF17 Polyclonal Antibody, Biotin (Product # 500-P152BT-1MG) is required. This biotinylated polyclonal antibody allows the detection of at least 2000-4000 pg/mL of Recombinant Human FGF-17.
Product Details
500-P152BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human FGF-17
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929501
Product Specific Information
AA Sequence of recombinant protein: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17. Anti-Human FGF-17-specific antibody was purified by affinity chromatography and then biotinylated.
Direct ELISA: To detect Human FGF-17 by direct ELISA (using 100 µL/well) a concentration of approximately 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 2000-4000 pg/mL of Recombinant Human FGF-17.
Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P152BT-1 mg will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development. This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-17; Fibroblast growth factor; Fibroblast growth factor 17
Gene Aliases: FGF-13; FGF-17; FGF17; HH20; UNQ161/PRO187
UniProt ID: (Human) O60258
Entrez Gene ID: (Human) 8822
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
type 1 fibroblast growth factor receptor binding
type 2 fibroblast growth factor receptor binding
growth factor activity
1-phosphatidylinositol-3-kinase activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
growth factor
intercellular signal molecule
MAPK cascade
signal transduction
cell-cell signaling
nervous system development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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