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Invitrogen
FGF23 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
FGF23 Antibody (PA5-46997) in Neu
Neutralization of FGF23 in NIH‚3T3 mouse embryonic fibroblast cell line. Samples were incubated in FGF23 polyclonal antibody (Product # PA5-46997). In the presence of Recombinant Mouse Klotho (5 µg/mL) and heparin (10 µg/mL), Recombinant Human FGF‚23 stimulates proliferation in the NIH‚3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF‚23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‚23 Antigen... View More
Product Details
PA5-46997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FGF-23 Tyr25-Ile251
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605534
Product Specific Information
In Western blots, approximately 25% cross-reactivity with recombinant mouse FGF-23 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.24 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The product of this gene inhibits renal tubular phosphate transport. This gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets, an inherited phosphate wasting disorder. Abnormally high level expression of this gene was found in oncogenic hypophosphatemic osteomalacia, a phenotypically similar disease caused by abnormal phosphate metabolism. Mutations in this gene have also been shown to cause familial tumoral calcinosis with hyperphosphatemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-23; Fibroblast growth factor; Fibroblast growth factor 23; Phosphatonin; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor
Gene Aliases: ADHR; FGF23; FGFN; HPDR2; HYPF; PHPTC; UNQ3027/PRO9828
UniProt ID: (Human) Q9GZV9
Entrez Gene ID: (Human) 8074
MAPK cascade
fibroblast growth factor receptor signaling pathway
regulation of phosphate transport
positive regulation of vitamin D 24-hydroxylase activity
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cell differentiation
negative regulation of bone mineralization
cellular phosphate ion homeostasis
response to magnesium ion
phosphatidylinositol-3-phosphate biosynthetic process
vitamin D catabolic process
positive regulation of GTPase activity
cellular response to leptin stimulus
negative regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
phosphatidylinositol phosphorylation
negative regulation of hormone secretion
phosphatidylinositol-mediated signaling
phosphate ion homeostasis
positive regulation of ERK1 and ERK2 cascade
cellular response to vitamin D
cellular response to interleukin-6
cellular response to parathyroid hormone stimulus
positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway
response to sodium phosphate
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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