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FGF4 Antibody (500-P158BT-25UG) in ELISA
Sandwich ELISA: To detect hFGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of FGF4 Polyclonal Antibody, Biotin (Product # 500-P158BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech FGF4 Polyclonal Antibody (Product # 500-P158-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFGF-4.
Product Details
500-P158BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human FGF-4
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929544
Product Specific Information
AA Sequence of recombinant protein: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-4. Anti-Human FGF-4-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hFGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-4 (500-P158) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFGF-4.
Western Blot: To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P158BT-1MG will be provided as 2 x 500 µg
Target Information
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3
Gene Aliases: FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; KS3
UniProt ID: (Human) P08620
Entrez Gene ID: (Human) 2249
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
growth factor
intercellular signal molecule
MAPK cascade
cartilage condensation
signal transduction
cell-cell signaling
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
mesenchymal cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
stem cell population maintenance
embryonic hindlimb morphogenesis
phosphatidylinositol-3-phosphate biosynthetic process
odontogenesis of dentin-containing tooth
negative regulation of apoptotic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of cell division
cranial suture morphogenesis
apoptotic process involved in morphogenesis
chondroblast differentiation
positive regulation of ERK1 and ERK2 cascade
regulation of endothelial cell chemotaxis to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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