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FGF9 Antibody (500-P66BT-25UG) in ELISA
Sandwich ELISA: To detect mFGF-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of FGF9 Polyclonal Antibody, Biotin (Product # 500-P66BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech FGF9 Polyclonal Antibody (Product # 500-P66-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mFGF-9.
Product Details
500-P66BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine FGF-9
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930693
Product Specific Information
AA Sequence of recombinant protein: PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine FGF-9. Anti-Murine FGF-9-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mFGF-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine FGF-9 (500-P66) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mFGF-9.
Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P66BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elbow knee synostosis; FGF; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; GAF; glia activating factor; Glia-activating factor; HBGF-9; M-FGF-9
Gene Aliases: Eks; Fgf-9; Fgf9
UniProt ID: (Mouse) P54130
Entrez Gene ID: (Mouse) 14180
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
osteoblast differentiation
positive regulation of mesenchymal cell proliferation
chondrocyte differentiation
cardiac left ventricle morphogenesis
cardiac ventricle development
protein import into nucleus
cell-cell signaling
multicellular organism development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
male gonad development
positive regulation of gene expression
cell differentiation
negative regulation of Wnt signaling pathway
male sex determination
lung development
embryonic limb morphogenesis
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of activin receptor signaling pathway
inner ear morphogenesis
positive regulation of MAPK cascade
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
regulation of timing of cell differentiation
embryonic digestive tract development
embryonic skeletal system development
positive regulation of epithelial cell proliferation
positive regulation of cell division
positive regulation of cardiac muscle cell proliferation
lung-associated mesenchyme development
vasculogenesis involved in coronary vascular morphogenesis
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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