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Product Details
31687
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1F8-1E4
Conjugate
Excitation/Emission Max
552/578 nm
View spectra
Form
Lyophilized
Concentration
1.7 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_908075
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Fluorophore: Tetramethyl rodamine 5 (and 6)-isothiocyanate (TRITC)
Amax = 550 nm; Emax = 570 nm
Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44
Product #31687 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical, immunoprecipitation and Western blot applications.
Restoration and Storage: Store product at 4°C until opened. Restore with 0.35 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C; or as an alternative, aliquot without glycerol and store at -80º or below, avoiding repeated freezing and thawing.
Country of Origin: USA
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fluorescein
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