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FLT3LG Antibody (500-P42-1MG) in ELISA
Sandwich ELISA: To detect Human Flt3-Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of FLT3LG Polyclonal Antibody (Product # 500-P42-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech FLT3LG Polyclonal Antibody, Biotin (Product # 500-P42BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Flt3-Ligand.
Product Details
500-P42-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Flt3-Ligand
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930534
Product Specific Information
AA Sequence of recombinant protein: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Flt3-Ligand. Anti-Human Flt3-Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human Flt3-Ligand matrix.
Sandwich ELISA: To detect Human Flt3-Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Flt3-Ligand (500-P42Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Flt3-Ligand.
Western Blot: To detect Human Flt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Flt3-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a Protein Coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Ras signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and cytokine activity. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Flt 3 ligand; Flt3 L; Flt3 ligand; Fms-related tyrosine kinase 3 ligand; H-FLT-3-L; M-FLT-3-L; SL cytokine
Gene Aliases: FL; FLT3L; FLT3LG
UniProt ID: (Human) P49771
Entrez Gene ID: (Human) 2323
positive regulation of protein phosphorylation
signal transduction
positive regulation of cell proliferation
lymphocyte differentiation
regulation of myeloid dendritic cell activation
positive regulation of natural killer cell differentiation
embryonic hemopoiesis
positive regulation of myoblast differentiation
positive regulation of cell cycle
positive regulation of transcription from RNA polymerase II promoter
homeostasis of number of cells within a tissue
positive regulation of cell proliferation in bone marrow
negative regulation of apoptotic process in bone marrow
positive regulation of osteoclast proliferation
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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