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FLT4 Antibody (CF806805) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT4 (RC214285, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT4. Positive lysates LY405313 (100 µg) and LC405313 (20 µg) can be purchased separately from OriGene.
Product Details
CF806805
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1G2
Immunogen
Human recombinant protein fragment corresponding to amino acids 1063-1363 of human FLT4 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLT-4; Fms-like tyrosine kinase 4; fms-related tyrosine kinase 4; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; VEGF R3; VEGFR-3
Gene Aliases: FLT-4; FLT4; FLT41; LMPH1A; PCL; VEGFR-3; VEGFR3
UniProt ID: (Human) P35916
Entrez Gene ID: (Human) 2324
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
vasculature development
lymph vessel development
lymphangiogenesis
sprouting angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
positive regulation of endothelial cell migration
peptidyl-tyrosine phosphorylation
cellular response to vascular endothelial growth factor stimulus
vascular endothelial growth factor signaling pathway
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of JNK cascade
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
blood vessel morphogenesis
regulation of blood vessel remodeling
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein kinase C signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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