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FNDC5 Antibody (PA5-121407) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of FNDC5 in SH-SY5Y cells using FNDC5 Polyclonal Antibody (Product # PA5-121407) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product Details
PA5-121407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Fibronectin type III domain-containing protein 5 protein (113-127aa)
Conjugate
Form
Liquid
Concentration
3.73 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2914979
Product Specific Information
Antibody tested in Indirect ELISA
Target Information
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2
Gene Aliases: FNDC5; FRCP2; irisin
UniProt ID: (Human) Q8NAU1
Entrez Gene ID: (Human) 252995
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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