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FOXJ1 Antibody (720226) in WB
Western blot analysis was performed on Nuclear extracts (30 µg lysate) of Caco-2 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3), RAW 264.7 (Lane 4) and Tissue extracts of Mouse lung (Lane 5), Mouse Heart (Lane 6) and Rat Heart (Lane 7). The blots were probed with Anti-FOXJ1 Rabbit Polyclonal Antibody (Product # 720226, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 45 kDa corresponding to FOXJ1 was observed acros... View More
Product Details
720226
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to human FOXJ1 (aa267-aa284, aa403-aa421)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2664304
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig
Target Information
Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity, through the repression of NF B activity. FOXJ1 also inhibits the humoral immune response in B cells. FOXJ1 deficiency in B cells results in spontaneous and accentuated germinal center formation and is implicated in the development of pathogenic autoantibodies and accentuated responses to immunizations. Abnormal expression of FOXJ1 may be associated with autoimmune diseases and/or other inflammatory diseases. FOXJ1 is also required for cilia formation and left-right axis determination because it increases calpastatin expression, a protein necessary for the ability of basal bodies to anchor to the apical cytoskeleton. FOXJ1 expression may function as an early marker of epithelial cell differentiation, recovery, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fork head homologue 4; Forkhead box protein J1; forkhead transcription factor HFH-4; forkhead-like 13; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HNF-3/forkhead homolog 4; MGC35202
Gene Aliases: FKHL-13; FKHL13; FOXJ1; HFH-4; HFH4
UniProt ID: (Human) Q92949, (Mouse) Q61660, (Rat) Q63247
Entrez Gene ID: (Human) 2302, (Mouse) 15223, (Rat) 116557
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
central tolerance induction
negative regulation of germinal center formation
positive regulation of central B cell tolerance induction
negative regulation of humoral immune response mediated by circulating immunoglobulin
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
humoral immune response
cell projection organization
actin cytoskeleton organization
cell differentiation
regulation of epithelial cell differentiation
negative regulation of NF-kappaB transcription factor activity
negative regulation of T cell differentiation in thymus
establishment of apical/basal cell polarity
negative regulation of T cell proliferation
cilium assembly
negative regulation of interleukin-6 biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of B cell activation
leukocyte migration
cilium morphogenesis
left/right pattern formation
activation of GTPase activity
positive regulation of lung ciliated cell differentiation
spermatogenesis
determination of left/right symmetry
pattern specification process
brain development
heart development
metanephric part of ureteric bud development
lung epithelium development
epithelium development
glomerular parietal epithelial cell development
regulation of transcription from RNA polymerase II promoter
response to organic cyclic compound
response to estradiol
cellular response to growth factor stimulus
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