Invitrogen

FOXO1 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-804

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(633) N V L P N Q S F P H S V K T(647) of human FOXO1a.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2106664

Product Specific Information

PA1-804 detects FoxO1a in human and rat samples.

PA1- 804 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~66 kDa protein representing FoxO1a in rat heart and lung protein extracts.

The PA1- 804 immunogen is a synthetic peptide corresponding to residues D(633) N V L P N Q S F P H S V K T(647) of human FOXO1a. This sequence is completely conserved in mouse and rat.

Fig. 1 shows a Western blot of FoxO1a in rat lung protein extract using PA1-804.

Target Information

FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A

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Gene Aliases: FKH1; FKHR; FOXO1; FOXO1A

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UniProt ID: (Human) Q12778, (Rat) G3V7R4

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Entrez Gene ID: (Human) 2308, (Rat) 84482

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding protein binding beta-catenin binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter blood vessel development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter autophagy apoptotic process cellular response to DNA damage stimulus insulin receptor signaling pathway cellular response to starvation positive regulation of autophagy cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade cellular response to oxidative stress regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter regulation of cell proliferation glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to hyperoxia cellular response to nitric oxide negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance regulation of nucleic acid-templated transcription regulation of neural precursor cell proliferation regulation of reactive oxygen species metabolic process temperature homeostasis cellular glucose homeostasis transcription, DNA-templated protein acetylation endocrine pancreas development fat cell differentiation negative regulation of transcription, DNA-templated enamel mineralization cellular response to hydrogen peroxide cellular response to cold cellular response to dexamethasone stimulus response to fluoride regulation of energy homeostasis
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