Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA5-14846
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
S.502.4
Immunogen
GST-fusion protein corresponding to carboxy-terminal residues of human FoxO1
Conjugate
Form
Liquid
Concentration
88 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10987316
Product Specific Information
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
It is not recommended to aliquot this antibody.
Target Information
FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A
Gene Aliases: Afxh; AI876417; FKH1; FKHR; Fkhr1; FOXO1; FOXO1A
UniProt ID: (Human) Q12778, (Rat) G3V7R4, (Mouse) Q9R1E0
Entrez Gene ID: (Human) 2308, (Rat) 84482, (Mouse) 56458
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator binding
chromatin binding
protein binding
beta-catenin binding
ubiquitin protein ligase binding
sequence-specific DNA binding
protein phosphatase 2A binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
autophagy
apoptotic process
cellular response to DNA damage stimulus
insulin receptor signaling pathway
cellular response to starvation
positive regulation of autophagy
cellular response to insulin stimulus
negative regulation of stress-activated MAPK cascade
cellular response to oxidative stress
regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter
regulation of cell proliferation
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of fat cell differentiation
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to hyperoxia
cellular response to nitric oxide
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
regulation of nucleic acid-templated transcription
regulation of neural precursor cell proliferation
regulation of reactive oxygen species metabolic process
transcription, DNA-templated
anatomical structure morphogenesis
cell differentiation
temperature homeostasis
cellular glucose homeostasis
protein acetylation
endocrine pancreas development
fat cell differentiation
negative regulation of transcription, DNA-templated
enamel mineralization
cellular response to hydrogen peroxide
cellular response to cold
cellular response to dexamethasone stimulus
response to fluoride
regulation of energy homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support