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Invitrogen
FOXO3A Recombinant Rabbit Monoclonal Antibody (5H17L19)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
FOXO3A Antibody (701847) in ICC/IF
For immunofluorescence analysis, serum starved (16 hours) SH-SY5Y cells were fixed and permeabilized for detection of endogenous Foxo3A using Anti- Foxo3A Recombinant Rabbit Monoclonal Antibody (Product # 701847, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Foxo3A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mou... View More
Product Details
701847
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H17L19
Immunogen
Peptides corresponding to Human FOXO3 (aa 298-312, 634-646)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633032
Product Specific Information
This antibody is predicted to react with Monkey, Sheep, Pig and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
USP48 is a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF6q21 protein; forkhead box O3A; Forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
Gene Aliases: AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3; FOXO3A
UniProt ID: (Human) O43524
Entrez Gene ID: (Human) 2309
core promoter binding
transcription cofactor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
protein kinase binding
chromatin DNA binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
ovulation from ovarian follicle
initiation of primordial ovarian follicle growth
antral ovarian follicle growth
oocyte maturation
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of translation
DNA damage response, signal transduction by p53 class mediator
tumor necrosis factor-mediated signaling pathway
cellular response to oxidative stress
glucose homeostasis
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
positive regulation of erythrocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
extrinsic apoptotic signaling pathway in absence of ligand
regulation of neural precursor cell proliferation
regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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