Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

49 Published Figures

64 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FOXP3 Antibody (11-4777-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD25 PE (Product # 12-0259-80) and subsequently fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00) and stained with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human Foxp3 FITC (right). Cells in the lymphocyte gate were used for analysis.

FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FOXP3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FOXP3 Antibody in Immunohistochemistry (IHC)

FOXP3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

FOXP3 Antibody in Radioimmune assays (RIA)

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis. {RE}

Product Details

11-4777-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 21 publications 21 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 36 publications 36 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Radioimmune Assays (RIA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

236A/E7

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149498

Product Specific Information

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776-40). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.

Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 236A/E7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Changes of T Lymphocyte Subsets in Peripheral Blood of Patients with Intermediate and Advanced Cervical Cancer before and after Nimotuzumab Combined with Chemoradiotherapy.

International archives of allergy and immunology

Teng F,Cui G,Qian L,Zhao L

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Thu Jan 05 00:00:00 EST 2023

Characterization of T Follicular Helper Cells and T Follicular Regulatory Cells in HIV-Infected and Sero-Negative Individuals.

Cells

Salvatore B,Resop RS,Gordon BR,Epeldegui M,Martinez-Maza O,Comin-Anduix B,Lam A,Wu TT,Uittenbogaart CH

11-4777-42 was used in Flow cytometry/Cell sorting to identify cytokines in peripheral blood T follicular helper cells and T follicular regulatory cells.

Thu Jan 12 00:00:00 EST 2023

Tumor microenvironment shows an immunological abscopal effect in patients with NSCLC treated with pembrolizumab-radiotherapy combination.

Journal for immunotherapy of cancer

van der Woude LL,Gorris MAJ,Wortel IMN,Creemers JHA,Verrijp K,Monkhorst K,Grünberg K,van den Heuvel MM,Textor J,Figdor CG,Piet B,Theelen WSME,de Vries IJM

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Sat Oct 01 00:00:00 EDT 2022

MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.

Immunity

Sugiura A,Andrejeva G,Voss K,Heintzman DR,Xu X,Madden MZ,Ye X,Beier KL,Chowdhury NU,Wolf MM,Young AC,Greenwood DL,Sewell AE,Shahi SK,Freedman SN,Cameron AM,Foerch P,Bourne T,Garcia-Canaveras JC,Karijolich J,Newcomb DC,Mangalam AK,Rabinowitz JD,Rathmell JC

11-4777-42 was used in Flow cytometry/Cell sorting to show that MTHFD2 is a metabolic checkpoint to integrate purine metabolism with pathogenic effector cell signaling and is a potential therapeutic target within 1C metabolism pathways.

Tue Jan 11 00:00:00 EST 2022

The transcription factor CREB1 is a mechanistic driver of immunogenicity and reduced HIV-1 acquisition following ALVAC vaccination.

Nature immunology

Tomalka JA,Pelletier AN,Fourati S,Latif MB,Sharma A,Furr K,Carlson K,Lifton M,Gonzalez A,Wilkinson P,Franchini G,Parks R,Letvin N,Yates N,Seaton K,Tomaras G,Tartaglia J,Robb ML,Michael NL,Koup R,Haynes B,Santra S,Sekaly RP

11-4777-42 was used in Flow Cytometry to validate CREB1 as a critical driver of vaccine efficacy and highlights that adjuvants that trigger CREB1 signaling may be critical for efficacious HIV-1 vaccines.

Fri Oct 01 00:00:00 EDT 2021

Analysis of Expression of Different Histone Deacetylases in Autoimmune Thyroid Disease.

The Journal of clinical endocrinology and metabolism

Sacristán-Gómez P,Serrano-Somavilla A,González-Amaro R,Martínez-Hernández R,Marazuela M

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.

Nature communications

Xydia M,Rahbari R,Ruggiero E,Macaulay I,Tarabichi M,Lohmayer R,Wilkening S,Michels T,Brown D,Vanuytven S,Mastitskaya S,Laidlaw S,Grabe N,Pritsch M,Fronza R,Hexel K,Schmitt S,Müller-Steinhardt M,Halama N,Domschke C,Schmidt M,von Kalle C,Schütz F,Voet T,Beckhove P

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Increased CD3⁺, CD8⁺, or FoxP3⁺ T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients.

Biology

Barbosa AM,Martinho O,Nogueira R,Campos J,Lobo L,Pinto H,Longatto-Filho A,Castro AG,Martins SF,Torrado E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Fri Aug 20 00:00:00 EDT 2021

Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.

International journal of molecular sciences

von Schoenfeld A,Bronsert P,Poc M,Fuller A,Filby A,Kraft S,Kurowski K,Sörensen K,Huber J,Pfeiffer J,Proietti M,Stehl V,Werner M,Seidl M

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Sat Dec 25 00:00:00 EST 2021

Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma.

Nature communications

Phillips D,Matusiak M,Gutierrez BR,Bhate SS,Barlow GL,Jiang S,Demeter J,Smythe KS,Pierce RH,Fling SP,Ramchurren N,Cheever MA,Goltsev Y,West RB,Khodadoust MS,Kim YH,Schürch CM,Nolan GP

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Thu Nov 18 00:00:00 EST 2021

Muscle-Directed Delivery of an AAV1 Vector Leads to Capsid-Specific T Cell Exhaustion in Nonhuman Primates and Humans.

Molecular therapy : the journal of the American Society of Gene Therapy

Gernoux G,Gruntman AM,Blackwood M,Zieger M,Flotte TR,Mueller C

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Wed Mar 04 00:00:00 EST 2020

Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.

EMBO reports

Court AC,Le-Gatt A,Luz-Crawford P,Parra E,Aliaga-Tobar V,Bátiz LF,Contreras RA,Ortúzar MI,Kurte M,Elizondo-Vega R,Maracaja-Coutinho V,Pino-Lagos K,Figueroa FE,Khoury M

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Wed Feb 05 00:00:00 EST 2020

Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.

Journal of cellular and molecular medicine

Zhang M,Liu Y,Chen J,Chen L,Meng J,Yang C,Yin S,Zhang X,Zhang L,Hao Z,Chen X,Liang C

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.

EBioMedicine

Todorova D,Zhang Y,Chen Q,Liu J,He J,Fu X,Xu Y

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells.

The journal of pathology. Clinical research

Silva MA,Triltsch N,Leis S,Kanchev I,Tan TH,Van Peel B,Van Kerckhoven M,Deschoolmeester V,Zimmermann J

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer.

Frontiers in physiology

Mi H,Gong C,Sulam J,Fertig EJ,Szalay AS,Jaffee EM,Stearns V,Emens LA,Cimino-Mathews AM,Popel AS

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Wed Nov 18 00:00:00 EST 2020

Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs.

Genes

Scherer A,Stephens VR,McGivney GR,Gutierrez WR,Laverty EA,Knepper-Adrian V,Dodd RD

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Sat May 23 00:00:00 EDT 2020

Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells.

Nature communications

Gómez-Aleza C,Nguyen B,Yoldi G,Ciscar M,Barranco A,Hernández-Jiménez E,Maetens M,Salgado R,Zafeiroglou M,Pellegrini P,Venet D,Garaud S,Trinidad EM,Benítez S,Vuylsteke P,Polastro L,Wildiers H,Simon P,Lindeman G,Larsimont D,Van den Eynden G,Velghe C,Rothé F,Willard-Gallo K,Michiels S,Muñoz P,Walzer T,Planelles L,Penninger J,Azim HA,Loi S,Piccart M,Sotiriou C,González-Suárez E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Thu Dec 10 00:00:00 EST 2020

Inhibitory activity of FOXP3+ regulatory T cells reveals high specificity for displaying immune tolerance in remission state rheumatoid arthritis.

Scientific reports

Kanjana K,Chevaisrakul P,Matangkasombut P,Paisooksantivatana K,Lumjiaktase P

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Nov 13 00:00:00 EST 2020

Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy.

Cell research

Zhang Y,Du X,Liu M,Tang F,Zhang P,Ai C,Fields JK,Sundberg EJ,Latinovic OS,Devenport M,Zheng P,Liu Y

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes.

Cell metabolism

Thompson PJ,Shah A,Ntranos V,Van Gool F,Atkinson M,Bhushan A

11-4777-42 was used in Flow cytometry/Cell sorting to show that during the natural history of type 1 diabetes in humans and the non-obese diabetic mouse model, a subset of beta cells acquires a senescence-associated secretory phenotype.

Tue May 07 00:00:00 EDT 2019

GM-CSF Promotes Antitumor Immunity by Inducing Th9 Cell Responses.

Cancer immunology research

Kim IK,Koh CH,Jeon I,Shin KS,Kang TS,Bae EA,Seo H,Ko HJ,Kim BS,Chung Y,Kang CY

11-4777-42 was used in Flow Cytometry to demonstrate that GM-CSF potentiates the efficacy of cancer vaccines through IL9-producing Th (Th9) cells.

Fri Mar 01 00:00:00 EST 2019

Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes.

Oncoimmunology

Triozzi PL,Schoenfield L,Plesec T,Saunthararajah Y,Tubbs RR,Singh AD

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Wed Feb 03 00:00:00 EST 2021

The immune system in sporadic inclusion body myositis patients is not compromised by blood-flow restricted exercise training.

Arthritis research & therapy

Jensen KY,Jacobsen M,Schrøder HD,Aagaard P,Nielsen JL,Jørgensen AN,Boyle E,Bech RD,Rosmark S,Diederichsen LP,Frandsen U

11-4777-42 was used in Immunohistochemistry (Frozen) to investigate the longitudinal effects of low-load blood flow restriction exercise on innate and adaptive immune markers in skeletal muscle from sIBM patients.

Wed Dec 18 00:00:00 EST 2019

Increased circulating regulatory T cells in medicated people with schizophrenia.

Psychiatry research

Kelly DL,Li X,Kilday C,Feldman S,Clark S,Liu F,Buchanan RW,Tonelli LH

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2018

Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report.

Frontiers in oncology

Roelofsen T,Wefers C,Gorris MAJ,Textor JC,Massuger LFAG,de Vries IJM,van Altena AM

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Wed Oct 04 00:00:00 EDT 2023

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

Oncotarget

Onion D,Isherwood M,Shridhar N,Xenophontos M,Craze ML,Day LJ,García-Márquez MA,Pineda RG,Reece-Smith AM,Saunders JH,Duffy JP,Argent RH,Grabowska AM

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Fri Jan 05 00:00:00 EST 2018

PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia.

Scientific reports

Lopatina T,Favaro E,Grange C,Cedrino M,Ranghino A,Occhipinti S,Fallo S,Buffolo F,Gaykalova DA,Zanone MM,Romagnoli R,Camussi G

11-4777 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory activity and protection from acute hindlimb ischaemia of extracellular vesicles released by platelet-derived growth factor-stimulated adipose-derived mesenchymal stem cells.

Tue Dec 04 00:00:00 EST 2018

MicroRNA-31 Targets Thymic Stromal Lymphopoietin in Mucosal Infiltrated CD4+ T Cells: A Role in Achieving Mucosal Healing in Ulcerative Colitis?

Inflammatory bowel diseases

Whiteoak SR,Claridge A,Balendran CA,Harris RJ,Gwiggner M,Bondanese VP,Erlandsson F,Hansen MB,Cummings JRF,Sanchez-Elsner T

11-4777-42 was used in Flow cytometry/Cell sorting to find that TSLP is reduced at both mRNA and protein levels in inflamed UC patients when compared with healthy subjects, in both whole biopsies and biopsy-isolated CD4+ CD25+ T cells.

Fri Oct 12 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Tue Nov 21 00:00:00 EST 2017

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

11-4777 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Radioimmune Assays

Mon May 15 00:00:00 EDT 2017

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

Oncoimmunology

Jiang X,Seo YD,Chang JH,Coveler A,Nigjeh EN,Pan S,Jalikis F,Yeung RS,Crispe IN,Pillarisetty VG

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Sat Nov 04 00:00:00 EDT 2023

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Tue May 16 00:00:00 EDT 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

TGF-β Affects the Differentiation of Human GM-CSF⁺ CD4⁺ T Cells in an Activation- and Sodium-Dependent Manner.

Frontiers in immunology

Éliás S,Schmidt A,Kannan V,Andersson J,Tegnér J

11-4777 was used in Flow cytometry/Cell sorting to study the environmental cues that promote differentiation of granulocyte-macrophage colony-stimulating factor producing T cells.

Wed Nov 20 00:00:00 EST 2019

The immune microenvironment of breast ductal carcinoma in situ.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Thompson E,Taube JM,Elwood H,Sharma R,Meeker A,Warzecha HN,Argani P,Cimino-Mathews A,Emens LA

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2016

Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.

International journal of molecular medicine

Liang DY,Hou YQ,Luo LJ,Ao L

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer.

Journal of translational medicine

Miyan M,Schmidt-Mende J,Kiessling R,Poschke I,de Boniface J

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry (Frozen)

Fri Jul 29 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Ghosh S,Taylor A,Chin M,Huang HR,Conery AR,Mertz JA,Salmeron A,Dakle PJ,Mele D,Cote A,Jayaram H,Setser JW,Poy F,Hatzivassiliou G,DeAlmeida-Nagata D,Sandy P,Hatton C,Romero FA,Chiang E,Reimer T,Crawford T,Pardo E,Watson VG,Tsui V,Cochran AG,Zawadzke L,Harmange JC,Audia JE,Bryant BM,Cummings RT,Magnuson SR,Grogan JL,Bellon SF,Albrecht BK,Sims RJ,Lora JM

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?

PloS one

Stikvoort A,Sundin M,Uzunel M,Gertow J,Sundberg B,Schaffer M,Mattsson J,Uhlin M

11-4777 was used in Flow cytometry/Cell sorting to study how the populations of NKT-cells differs following hematopoietic stem cell transplantation according to whether a patient retains any of their own immune cells or not.

Mon Jul 17 00:00:00 EDT 2017

Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2^V617F allelic burden.

Experimental hematology & oncology

Kovacsovics-Bankowski M,Kelley TW,Efimova O,Kim SJ,Wilson A,Swierczek S,Prchal J

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

11-4777 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Skogberg G,Lundberg V,Lindgren S,Gudmundsdottir J,Sandström K,Kämpe O,Annerén G,Gustafsson J,Sunnegårdh J,van der Post S,Telemo E,Berglund M,Ekwall O

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Mon Sep 01 00:00:00 EDT 2014

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Wed Jul 30 00:00:00 EDT 2014

Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.

European journal of immunology

Dokić J,Tomić S,Marković M,Milosavljević P,Colić M

11-4777 was used in Flow cytometry/Cell sorting to investigate whether mesenchymal stem cells from periapical lesions modulate the development and functions of dendritic cells.

Mon Jul 01 00:00:00 EDT 2013

HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.

Oncoimmunology

Schmidt HH,Ge Y,Hartmann FJ,Conrad H,Klug F,Nittel S,Bernhard H,Domschke C,Schuetz F,Sohn C,Beckhove P

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.

Journal of immunology (Baltimore, Md. : 1950)

Sucher R,Fischler K,Oberhuber R,Kronberger I,Margreiter C,Ollinger R,Schneeberger S,Fuchs D,Werner ER,Watschinger K,Zelger B,Tellides G,Pilat N,Pratschke J,Margreiter R,Wekerle T,Brandacher G

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Sun Jan 01 00:00:00 EST 2012

Th17/Treg imbalance in patients with liver cystic echinococcosis.

Parasite immunology

Tuxun T,Wang JH,Lin RY,Shan JY,Tai QW,Li T,Zhang JH,Zhao JM,Wen H

11-4777-42 was used in Flow cytometry/Cell sorting to indicate that Th17/Treg functional imbalance exists in patients with chronic cystic echinococcosis, suggesting a potential role for Th17/Treg imbalance in the pathogenesis of immune evasion in echinococcosis.

Thu Nov 01 00:00:00 EDT 2012

A pilot study of MUC-1/CEA/TRICOM poxviral-based vaccine in patients with metastatic breast and ovarian cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mohebtash M,Tsang KY,Madan RA,Huen NY,Poole DJ,Jochems C,Jones J,Ferrara T,Heery CR,Arlen PM,Steinberg SM,Pazdur M,Rauckhorst M,Jones EC,Dahut WL,Schlom J,Gulley JL

11-4777 was used in Flow cytometry/Cell sorting to examine the clinical response to PANVAC in metastatic breast and ovarian cancer patients.

Tue Nov 15 00:00:00 EST 2011

Histone/protein deacetylase inhibitors increase suppressive functions of human FOXP3+ Tregs.

Clinical immunology (Orlando, Fla.)

Akimova T,Ge G,Golovina T,Mikheeva T,Wang L,Riley JL,Hancock WW

11-4777 was used in Flow cytometry/Cell sorting to investigate the effect of HDAC inhibitors on Tregs.

Wed Sep 01 00:00:00 EDT 2010

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 EDT 2010

The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Law JP,Hirschkorn DF,Owen RE,Biswas HH,Norris PJ,Lanteri MC

11-4777 was used in Flow cytometry/Cell sorting to develop a multiparameter flow cytometric panel with Foxp3 for optimal identification of Tregs.

Tue Dec 01 00:00:00 EST 2009

Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Kasprzycka M,Zhang Q,Witkiewicz A,Marzec M,Potoczek M,Liu X,Wang HY,Milone M,Basu S,Mauger J,Choi JK,Abrams JT,Hou JS,Rook AH,Vonderheid E,Woetmann A,Odum N,Wasik MA

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Immunohistochemistry

Fri Aug 15 00:00:00 EDT 2008

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

Thu May 15 00:00:00 EDT 2008

Chemokine and cytokine mediated loss of regulatory T cells in lymph nodes during pathogenic simian immunodeficiency virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Qin S,Sui Y,Soloff AC,Junecko BA,Kirschner DE,Murphey-Corb MA,Watkins SC,Tarwater PM,Pease JE,Barratt-Boyes SM,Reinhart TA

11-4777 was used in Flow cytometry/Cell sorting to suggest mechanisms for SIV infection driven loss of lymphoid Treg cell in macaque model and possible therapeutic intervention for HIV-1-infected individuals.

Tue Apr 15 00:00:00 EDT 2008

IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

Blood

Ahmadzadeh M,Rosenberg SA

Published figure using FOXP3 monoclonal antibody (Product # 11-4777-42) in Flow Cytometry

Wed Mar 15 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1

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Entrez Gene ID: (Human) 50943, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

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FOXP3 Monoclonal Antibody (236A/E7), FITC, eBioscience™

FOXP3 Antibody (11-4777-42) in Flow

Product Details

11-4777-42

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