Invitrogen

FOXP3 Recombinant Rabbit Monoclonal Antibody (5H10L18)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

11 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FOXP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

FOXP3 Antibody (700914) in IHC (P)

Immunohistochemistry analysis of FOXP3 showing staining in the nucleus of paraffin-embedded mouse spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, wash... View More

FOXP3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

FOXP3 Antibody — Figure 4 Cambogin prevents Foxp3 loss in LPS treated cells. (A) Flag-Foxp3 or (B) human Treg cells were treated with LPS (1 µg·mL−1) for 24 h in the absence or after cambogin pretreatment for 1 h. The cells were harvested for Western blotting as indicated. Data were quantified from five independent experiments. The relative protein levels were normalized to GAPDH by using ImageJ software. {TM}

Product Details

700914

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

5H10L18

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532349

Product Specific Information

This antibody is predicted to react with bovine, feline, porcine and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor cell-based vaccine contributes to local tumor irradiation by eliciting a tumor model-dependent systemic immune response.

Frontiers in immunology

Remic T,Sersa G,Levpuscek K,Lampreht Tratar U,Ursic Valentinuzzi K,Cör A,Kamensek U

700914 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that vaccine elicits an immune response and contributes to the antitumor efficacy of tumor irradiation; this interaction is multi-faceted and appears to be dependent on the tumor immunogenicity.

Fri Sep 23 00:00:00 EDT 2022

A Biological Circuit Involving Mef2c, Mef2d, and Hdac9 Controls the Immunosuppressive Functions of CD4+Foxp3+ T-Regulatory Cells.

Frontiers in immunology

Di Giorgio E,Wang L,Xiong Y,Christensen LM,Akimova T,Han R,Samanta A,Trevisanut M,Brancolini C,Beier UH,Hancock WW

700914 was used in Western Blot to report that as with Mef2d, the deletion of Mef2c in Tregs switches off the expression of Il10 and Icos and leads to enhanced antitumor immunity in syngeneic models of lung cancer.

Fri Dec 17 00:00:00 EST 2021

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

700914 was used in Western Blotting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 EST 2020

Inhibiting the coregulator CoREST impairs Foxp3+ Treg function and promotes antitumor immunity.

The Journal of clinical investigation

Xiong Y,Wang L,Di Giorgio E,Akimova T,Beier UH,Han R,Trevisanut M,Kalin JH,Cole PA,Hancock WW

700914 was used in Western Blotting to analyse the role in Tregs of the evolutionarily conserved CoREST complex, consisting of a scaffolding protein, Rcor1 or Rcor2, plus Hdac1 or Hdac2 and Lsd1 enzymes.

Wed Apr 01 00:00:00 EDT 2020

Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID.

Molecular therapy. Methods & clinical development

Garcia-Perez L,van Eggermond M,van Roon L,Vloemans SA,Cordes M,Schambach A,Rothe M,Berghuis D,Lagresle-Peyrou C,Cavazzana M,Zhang F,Thrasher AJ,Salvatori D,Meij P,Villa A,Van Dongen JJM,Zwaginga JJ,van der Burg M,Gaspar HB,Lankester A,Staal FJT,Pike-Overzet K

Published figure using FOXP3 recombinant monoclonal antibody (Product # 700914) in Immunohistochemistry

Fri Jun 12 00:00:00 EDT 2020

Dataset on the identification of a prognostic radio-immune signature in surgically resected Non Small Cell Lung Cancer.

Data in brief

Mazzaschi G,Milanese G,Pagano P,Madeddu D,Gnetti L,Trentini F,Falco A,Frati C,Lorusso B,Lagrasta C,Minari R,Ampollini L,Silva M,Sverzellati N,Quaini F,Roti G,Tiseo M

700914 was used in Immunohistochemistry to further investigate the possibility that immune microenvironmental factors in surgically resected Non Small Cell Lung Cancer may impact on clinical outcome and treatment response.

Sat Aug 01 00:00:00 EDT 2020

Dysfunctional and Proinflammatory Regulatory T-Lymphocytes Are Essential for Adverse Cardiac Remodeling in Ischemic Cardiomyopathy.

Circulation

Bansal SS,Ismahil MA,Goel M,Zhou G,Rokosh G,Hamid T,Prabhu SD

700914 was used in Immunohistochemistry to report the pathogenic role of pro-inflammatory and anti-angiogenic Tregs in chronic ischemic heart failure to promote immune activation and pathological left ventricular remodeling.

Tue Jan 08 00:00:00 EST 2019

Plant Virus-Like Particle In Situ Vaccine for Intracranial Glioma Immunotherapy.

Cancers

Kerstetter-Fogle A,Shukla S,Wang C,Beiss V,Harris PLR,Sloan AE,Steinmetz NF

700914 was used in Immunohistochemistry to study the efficacy of nanopartical platform technology to instigate a potent immune response against intracranial glioma.

Wed Apr 10 00:00:00 EDT 2019

Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function.

British journal of pharmacology

Lu Y,Kim NM,Jiang YW,Zhang H,Zheng D,Zhu FX,Liang R,Li B,Xu HX

700914 was used in Western Blotting to investigate the mechanisms by which cambogin suppresses colitis by enhancing regulatory T cell stability and function.

Sun Apr 01 00:00:00 EDT 2018

Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function.

British journal of pharmacology

Lu Y,Kim NM,Jiang YW,Zhang H,Zheng D,Zhu FX,Liang R,Li B,Xu HX

700914 was used in Western Blotting to investigate the mechanisms by which cambogin suppresses colitis by enhancing regulatory T cell stability and function.

Sun Apr 01 00:00:00 EDT 2018

Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function.

British journal of pharmacology

Lu Y,Kim NM,Jiang YW,Zhang H,Zheng D,Zhu FX,Liang R,Li B,Xu HX

700914 was used in Western Blotting to investigate the mechanisms by which cambogin suppresses colitis by enhancing regulatory T cell stability and function.

Sun Apr 01 00:00:00 EDT 2018

Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function.

British journal of pharmacology

Lu Y,Kim NM,Jiang YW,Zhang H,Zheng D,Zhu FX,Liang R,Li B,Xu HX

700914 was used in Western Blotting to investigate the mechanisms by which cambogin suppresses colitis by enhancing regulatory T cell stability and function.

Sun Apr 01 00:00:00 EDT 2018

Potentiation of PD-L1 blockade with a potency-matched dual cytokine-antibody fusion protein leads to cancer eradication in BALB/c-derived tumors but not in other mouse strains.

Cancer immunology, immunotherapy : CII

De Luca R,Neri D

700914 was used in Immunofluorescence in immunohistochemistry on frozen tissues to study the therapeutic activity of antibody-cytokine fusion protein IL2 in immunocompetent mouse cancer models.

Sat Sep 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; scurfin; sf; XPID

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UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding nucleic acid binding DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Recombinant Rabbit Monoclonal Antibody (5H10L18)

FOXP3 Antibody (700914) in IHC (P)

Product Details

700914

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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