Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
FXN Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
FXN Antibody (PA5-95829) in ICC/IF
Immunofluorescence analysis of Frataxin, mitochondrial was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FXN Polyclonal Antibody (Product # PA5-95829) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product... View More
Product Details
PA5-95829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin (NP_0001352)
Conjugate
Form
Liquid
Concentration
1.76 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807631
Product Specific Information
Immunogen sequence: LRTDIDATCT PRRASSNQRG LNQIWNVKKQ SVYLMNLRKS GTLGHPGSLD ETTYERLAEE TLDSLAEFFE DLADKPYTFE DYDVSFGSGV LTVKLGGDLG TYVINKQTPN KQIWLSSPSS GPKRYDWTGK NWVYSHDGVS LHELLAAELT KALKTKLDLS SLAYSGKDA; Positive Samples: K-562, U-87MG, Jurkat, Raji, DU-145, Mouse kidney, Mouse liver, Mouse heart; Cellular Location: Cytoplasm, Mitochondrion
Target Information
FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Frataxin, mitochondrial; Friedreich ataxia; Friedreich ataxia protein; Fxn
Gene Aliases: CyaY; FA; FARR; FRDA; FXN; RGD1565754; X25
UniProt ID: (Human) Q16595, (Mouse) O35943
Entrez Gene ID: (Human) 2395, (Rat) 499335, (Mouse) 14297
oxidative phosphorylation
heme biosynthetic process
ion transport
cellular iron ion homeostasis
mitochondrion organization
adult walking behavior
positive regulation of cell proliferation
aerobic respiration
embryo development ending in birth or egg hatching
response to iron ion
regulation of ferrochelatase activity
protein autoprocessing
iron incorporation into metallo-sulfur cluster
proprioception
positive regulation of cell growth
negative regulation of multicellular organism growth
negative regulation of apoptotic process
positive regulation of catalytic activity
small molecule metabolic process
negative regulation of organ growth
positive regulation of lyase activity
cellular response to hydrogen peroxide
negative regulation of release of cytochrome c from mitochondria
positive regulation of succinate dehydrogenase activity
positive regulation of aconitate hydratase activity
response to organic cyclic compound
iron-sulfur cluster assembly
response to drug
positive regulation of axon extension
positive regulation of transferase activity
positive regulation of oxidoreductase activity
oxidation-reduction process
transport
iron ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support