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FZD4 Antibody (PA5-47228) in IHC (F)
Immunohistochemical analysis of FZD4 in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11). Samples were incubated in FZD4 polyclonal antibody (Product # PA5-47228) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma memb... View More
Product Details
PA5-47228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4 Phe37-Glu180
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607400
Product Specific Information
In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-8 and less than 2% cross-reactivity with rmFrizzled-3 and rmFrizzled-7 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
FZD4 is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. Frizzled 4 is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. Transcripts have also been identified in liver, kidney, pancreas, spleen, and fetal lung, and in smaller amounts in placenta, adult lung, prostate, testis, colon, and fetal brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD344; CD344 antigen; Frizzled-4; Fz-4; mFz4; MGC34390
Gene Aliases: Fz4; Fzd4
UniProt ID: (Mouse) Q61088
Entrez Gene ID: (Mouse) 14366
signal transducer activity
transmembrane signaling receptor activity
G-protein coupled receptor activity
protein binding
Wnt-protein binding
cytokine binding
PDZ domain binding
ubiquitin protein ligase binding
protein homodimerization activity
Wnt-activated receptor activity
protein heterodimerization activity
transmembrane signal receptor
luteinization
blood vessel development
vasculogenesis
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
multicellular organism development
sensory perception of sound
negative regulation of cell-substrate adhesion
Wnt signaling pathway
regulation of vascular endothelial growth factor receptor signaling pathway
locomotion involved in locomotory behavior
substrate adhesion-dependent cell spreading
extracellular matrix-cell signaling
non-canonical Wnt signaling pathway
progesterone secretion
positive regulation of JUN kinase activity
positive regulation of transcription, DNA-templated
positive regulation of sequence-specific DNA binding transcription factor activity
canonical Wnt signaling pathway
retina vasculature morphogenesis in camera-type eye
cerebellum vasculature morphogenesis
retinal blood vessel morphogenesis
Performance Guarantee
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