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Fe65 Antibody (PA5-148221) in ICC/IF
Immunocytochemistry analysis of Fe65 in Hela cells. Samples were incubated with Fe65 polyclonal antibody (Product # PA5-148221) using a dilution of 1:1,000 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus. Positive staining was localized to Nucleus.
Product Details
PA5-148221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human FE65/APBB1
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein, transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants have been described for this gene but some of their full length sequence is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); Amyloid beta precursor protein binding family B member 1; Amyloid-beta A4 precursor protein-binding family B member 1; Protein Fe65; stat-like protein
Gene Aliases: APBB1; FE65; MGC:9072; RIR
UniProt ID: (Human) O00213
Entrez Gene ID: (Human) 322
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
cellular response to DNA damage stimulus
cell cycle arrest
signal transduction
axonogenesis
response to iron ion
positive regulation of neuron projection development
negative regulation of cell growth
positive regulation of apoptotic process
histone H4 acetylation
positive regulation of DNA repair
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of protein secretion
negative regulation of thymidylate synthase biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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