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Ferritin Antibody (MA5-14694) in ELISA
Sandwich ELISA of Ferritin was performed by coating wells of a 96-well plate with 100 µL of an Ferritin mouse monoclonal antibody (Product # MA5-14694) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382) overnight at 4C. Wells were blocked with 100 µL of StartingBlock T20 (TBS) Blocking Buffer (Product # 28382) for 2 hours, and 100 µL of recombinant human ferritin light polypeptide (FTL) standards ranging from 1.6-1000 ng/mL (A) or 100 µL of diluted normal human serum or diluted normal human plasma (B) w... View More
Product Details
MA5-14694
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
321B7
Immunogen
Human liver ferritin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981279
Product Specific Information
MA5-14694 detects Ferritin in ELISA, IP, IF, and RIA applications and shows reactivity to human samples. The MA5-14694 Immunogen is human liver ferritin. MA5-14694 detects ferritin which has a predicted molecular weight of approximately 19kDa
Product MA514694 is a smaller package size of MIF2505 (formerly sold as a Seradyn product).
Target Information
Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; Ferritin heavy chain; Ferritin L subunit; ferritin L-chain; Ferritin light chain; ferritin light polypeptide-like 3; ferritin, heavy polypeptide 1; ferritin, light polypeptide; frih; fril; L apoferritin; MGC71996; placenta immunoregulatory factor; proliferation-inducing protein 15
Gene Aliases: FHC; FTH; FTH1; FTHL6; FTL; HFE5; LFTD; NBIA3; OK/SW-cl.84; PIG15; PLIF
UniProt ID: (Human) P02794, (Human) P02792
Entrez Gene ID: (Human) 2495, (Human) 2512
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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