Proteintech

Fibronectin Monoclonal Antibody (1G10F9)

Fibronectin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

Fibronectin Antibody (66042-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-IG (Fibronectin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibronectin Antibody in Western Blot (WB)

Fibronectin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66042-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:12,000

View 46 publications 46 publications

Immunohistochemistry (IHC)

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:300-1:1200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 12 publications 12 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G10F9

Immunogen

Fibronectin Fusion Protein Ag8016 (2177-2386 aa encoded by BC005858)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DQQRHKVRE EVVTVGNSVN EGLNQPTDDS CFDPYTVSHY AVGDEWERMS ESGFKLLCQC LGFGSGHFRC DSSRWCHDNG VNYKIGEKWD RQGENGQMMS CTCLGNGKGE FKCDPHEATC YDDGKTYHVG EQWQKEYLGA ICSCTCFGGQ RGWRCDNCRR PGGEPSPEGT TGQSYNQYSQ RYHQRTNTNV NCPIECFMPL DVQADREDSR E (2177-2386 aa encoded by BC005858)

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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