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Fibronectin Antibody (MA5-17075) in ICC/IF
Immunofluorescence analysis of Fibronectin was performed using 70% confluent log phase Hep G2 cells and in HepG2 cells treated with PTI. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Fibronectin Monoclonal Antibody (2F4) (Product # MA5-17075) at 1:100 ... View More
Product Details
MA5-17075
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F4
Immunogen
Purified recombinant fragment of human FN1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2538546
Product Specific Information
MA5-17075 targets FN1 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.
The MA5-17075 immunogen is purified recombinant fragment of human FN1 expressed in E. Coli.
MA5-17075 detects FN1 which has a predicted molecular weight of approximately 262.6kDa.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor
Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF
UniProt ID: (Human) P02751
Entrez Gene ID: (Human) 2335
angiogenesis
cell activation
regulation of protein phosphorylation
platelet degranulation
response to ischemia
acute-phase response
cell-substrate junction assembly
cell adhesion
calcium-independent cell-matrix adhesion
positive regulation of cell proliferation
glial cell migration
regulation of cell shape
response to wounding
response to ozone
positive regulation of gene expression
positive regulation of peptidase activity
peptide cross-linking
extracellular matrix disassembly
extracellular matrix organization
integrin activation
substrate adhesion-dependent cell spreading
cellular response to vascular endothelial growth factor stimulus
endodermal cell differentiation
cellular response to platelet-derived growth factor stimulus
wound healing
negative regulation of apoptotic process
positive regulation of axon extension
positive regulation of fibroblast proliferation
leukocyte migration
positive regulation of chemotaxis
response to glucocorticoid
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to mercury ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to prostaglandin E stimulus
cellular response to transforming growth factor beta stimulus
cellular response to BMP stimulus
positive regulation of substrate-dependent cell migration, cell attachment to substrate
negative regulation of transforming growth factor-beta secretion
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