Invitrogen

Fluorescein/Oregon Green Polyclonal Antibody, HRP

Fluorescein/Oregon Green Antibody in Immunocytochemistry (ICC/IF)

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Fluorescein/Oregon Green Antibody (A-21253) in ICC/IF

Nuclear and nonnuclear incorporation of 5-bromo-2'-deoxyuridine in live cells. Bovine pulmonary arterial endothelial (BPAE) cells were labeled with 5-bromo-2'-deoxyuridine (BrdU, Product # B23151) applied at a concentration of 10 µM for 30 minutes. After fixation with 4% formaldehyde in phosphate-buffered saline for 30 minutes, chromatin was denatured by treatment with 2 M HCl for 20 minutes. Incorporated B... View More

Fluorescein/Oregon Green Antibody in Immunohistochemistry (IHC)

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Product Details

A-21253

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fluorescein/Oregon Green

Conjugate

HRP HRP HRP

Form

Lyophilized

Purification

IgG fraction

Storage buffer

0.1M potassium phosphate, pH 8

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2535819

Product Specific Information

Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.

Store product at -20°C until opened. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.

Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.

Target Information

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of ATM kinase rescues planarian regeneration after lethal radiation.

EMBO reports

Shiroor DA,Wang KT,Sanketi BD,Tapper JK,Adler CE

A-21253 was used in Inhibition Assays to investigate if in planarians, Ataxia-Telangiectasia Mutated promotes radiation-induced apoptosis.

Thu May 04 00:00:00 EDT 2023

A genetically identified population of layer 4 neurons in auditory cortex that contributes to pre-pulse inhibition of the acoustic startle response.

Frontiers in neural circuits

Weible AP,Yavorska I,Narayanan A,Wehr M

A-21253 was used in In vitro experiments to demonstrate that cortical Gpr26 neurons are both necessary and sufficient for top-down modulation of the acoustic startle reflex, and are thus likely to be involved in sound detection.

Wed Sep 28 00:00:00 EDT 2022

Planarian stem cells sense the identity of the missing pharynx to launch its targeted regeneration.

eLife

Bohr TE,Shiroor DA,Adler CE

A-21253 was used in Immunohistochemistry-immunofluorescence to study tissue regeneration mechanisms utilised by planarian flatworms to different injury types in varying tissues.

Tue Jun 22 00:00:00 EDT 2021

A Layer 3→5 Circuit in Auditory Cortex That Contributes to Pre-pulse Inhibition of the Acoustic Startle Response.

Frontiers in neural circuits

Weible AP,Yavorska I,Kayal D,Duckler U,Wehr M

Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-21253) in Immunohistochemistry

Mon Oct 04 00:00:00 EDT 2021

Functional properties of stellate cells in medial entorhinal cortex layer II.

eLife

Rowland DC,Obenhaus HA,Skytøen ER,Zhang Q,Kentros CG,Moser EI,Moser MB

A-21253 was used in Immunohistochemistry-immunofluorescence to identify stellate cells and characterise their firing properties in freely moving mice.

Fri Sep 14 00:00:00 EDT 2018

A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance.

Nature communications

Ageta-Ishihara N,Yamazaki M,Konno K,Nakayama H,Abe M,Hashimoto K,Nishioka T,Kaibuchi K,Hattori S,Miyakawa T,Tanaka K,Huda F,Hirai H,Hashimoto K,Watanabe M,Sakimura K,Kinoshita M

A-21253 was used in Immunohistochemistry to study whether a CDC42EP4/septin-based glial scaffold facilitates perisynaptic localisation of GLAST and optimises the efficiency of glutamate-buffering and clearance.

Thu Dec 10 00:00:00 EST 2015

Mitochondrial dynamics regulate growth cone motility, guidance, and neurite growth rate in perinatal retinal ganglion cells in vitro.

Investigative ophthalmology & visual science

Steketee MB,Moysidis SN,Weinstein JE,Kreymerman A,Silva JP,Iqbal S,Goldberg JL

A-21253 was used in Immunocytochemistry to suggest that mitochondria locally modulate signalling in the distal neurite and growth cone to affect neurite growth direction and rate.

Tue Oct 30 00:00:00 EDT 2012

MicroRNA-146a: a key regulator of astrocyte-mediated inflammatory response.

PloS one

Iyer A,Zurolo E,Prabowo A,Fluiter K,Spliet WG,van Rijen PC,Gorter JA,Aronica E

A-21253 was used in immunohistochemistry - paraffin section to assess the role of miR-46a in the modulation of astrocyte-mediated inflammation.

Tue Mar 05 00:00:00 EST 2013

Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain.

Neural development

Lauter G,Söll I,Hauptmann G

A-21253 was used in immunohistochemistry to discuss methods to map overlapping and abutting regulatory gene expression domains.

Tue Apr 05 00:00:00 EDT 2011

Enhancement of peptide coupling to hydroxyapatite and implant osseointegration through collagen mimetic peptide modified with a polyglutamate domain.

Biomaterials

Culpepper BK,Phipps MC,Bonvallet PP,Bellis SL

A-21253 was used in ELISA to explore ways to optimize hydroxyapatite substrates

Wed Dec 01 00:00:00 EST 2010

Induction of sodium channel Na(x) (SCN7A) expression in rat and human hippocampus in temporal lobe epilepsy.

Epilepsia

Gorter JA,Zurolo E,Iyer A,Fluiter K,van Vliet EA,Baayen JC,Aronica E

A-21253 was used in immunohistochemistry - paraffin section to determine the expression and localization of SCN7A mRNA and protein in normal and epileptic rat and human hippocampus

Wed Sep 01 00:00:00 EDT 2010

Expression pattern of miR-146a, an inflammation-associated microRNA, in experimental and human temporal lobe epilepsy.

The European journal of neuroscience

Aronica E,Fluiter K,Iyer A,Zurolo E,Vreijling J,van Vliet EA,Baayen JC,Gorter JA

A-21253 was used in immunohistochemistry - paraffin section to determine the expression and cellular distribution of miRNA-146a in a rat and human temporal lobe epilepsy

Mon Mar 01 00:00:00 EST 2010

tRNA splicing endonuclease mutations cause pontocerebellar hypoplasia.

Nature genetics

Budde BS,Namavar Y,Barth PG,Poll-The BT,Nürnberg G,Becker C,van Ruissen F,Weterman MA,Fluiter K,te Beek ET,Aronica E,van der Knaap MS,Höhne W,Toliat MR,Crow YJ,Steinling M,Voit T,Roelenso F,Brussel W,Brockmann K,Kyllerman M,Boltshauser E,Hammersen G,Willemsen M,Basel-Vanagaite L,Krägeloh-Mann I,de Vries LS,Sztriha L,Muntoni F,Ferrie CD,Battini R,Hennekam RC,Grillo E,Beemer FA,Stoets LM,Wollnik B,Nürnberg P,Baas F

A-21253 was used in immunohistochemistry - paraffin section to report that RNA processing contributes to pontocerebellar hypoplasia

Mon Sep 01 00:00:00 EDT 2008

The conserved microRNA miR-8 tunes atrophin levels to prevent neurodegeneration in Drosophila.

Cell

Karres JS,Hilgers V,Carrera I,Treisman J,Cohen SM

A-21253 was used in immunohistochemistry to determine the consequences of mutating miR-8 in Drosophila

Fri Oct 05 00:00:00 EDT 2007

Enzyme-based visualization of receptor-ligand binding in tissues.

Laboratory investigation; a journal of technical methods and pathology

Montet X,Yuan H,Weissleder R,Josephson L

A-21253 was used in immunohistochemistry to analyze receptor-ligand binding in tissues by enzyme-based visualization

Mon May 01 00:00:00 EDT 2006

Animal MicroRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution.

Cell

Stark A,Brennecke J,Bushati N,Russell RB,Cohen SM

A-21253 was used in Immunohistochemistry-immunofluorescence to study the impact of short 3'UTRs on gene regulation by microRNAs.

Fri Dec 16 00:00:00 EST 2005

The effect of the addition of a polyglutamate motif to RGD on peptide tethering to hydroxyapatite and the promotion of mesenchymal stem cell adhesion.

Biomaterials

Sawyer AA,Weeks DM,Kelpke SS,McCracken MS,Bellis SL

A-21253 was used in ELISA to compare unmodified RGD with polyacidic-conjugated RGD

Thu Dec 01 00:00:00 EST 2005

Multiplex detection of RNA expression in Drosophila embryos.

Science (New York, N.Y.)

Kosman D,Mizutani CM,Lemons D,Cox WG,McGinnis W,Bier E

A-21253 was used to develop a fluorescence-based, multiplex in situ hybridization method to detect RNA transcripts

Fri Aug 06 00:00:00 EDT 2004

The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induces apoptosis in mouse nigrostriatal glia. Relevance to nigral neuronal death and striatal neurochemical changes.

The Journal of biological chemistry

Serra PA,Sciola L,Delogu MR,Spano A,Monaco G,Miele E,Rocchitta G,Miele M,Migheli R,Desole MS

A-21253 was used in immunohistochemistry - paraffin section to assess the effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treatment using mice

Fri Sep 13 00:00:00 EDT 2002

Chromogenic in situ hybridization: a practical alternative for fluorescence in situ hybridization to detect HER-2/neu oncogene amplification in archival breast cancer samples.

The American journal of pathology

Tanner M,Gancberg D,Di Leo A,Larsimont D,Rouas G,Piccart MJ,Isola J

A-21253 was used in immunohistochemistry - paraffin section to discuss new methods to detect HER-2/neu gene copies

Wed Nov 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: FITC

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Fluorescein/Oregon Green Polyclonal Antibody, HRP

Fluorescein/Oregon Green Antibody (A-21253) in ICC/IF

Product Details

A-21253

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Inhibition Assays (IA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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