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Fra1 Antibody (PA5-120908) in WB
Western blot analysis of Fra1 in extracts of HeLa cells. Samples were incubated with Fra1 Polyclonal antibody (Product # PA5-120908) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120908
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FOSL1 (NP_005429.1).
Conjugate
Form
Liquid
Concentration
2.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914480
Product Specific Information
Positive test controls include: HeLa, C6. The target is usually found in the following locations: Nucleus.
Immunogen sequence: MFRDFGEPGP SSGNGGGYGG PAQPPAAAQA AQQKFHLVPS INTMSGSQEL QWMVQPHFLG PSSYPRPLTY PQYSPPQPRP GVIRALGPPP GVRRRPCEQI
Target Information
The v-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. Members of the c-Fos gene family, including c-Fos, Fos B, Fra-1 and Fra-2, encode nuclear phosphoproteins that are rapidly and transiently induced by a variety of agents and function as transcriptional regulators for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers that bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FOS like 1, AP-1 trancription factor subunit; FOS like antigen 1; FOS-like antigen 1; FOS-like antigen-1; Fos-related antigen 1; FRA-1; HGNC:13718
Gene Aliases: AW538199; FOSL1; FRA; Fra-1; FRA1
UniProt ID: (Human) P15407, (Mouse) P48755, (Rat) P10158
Entrez Gene ID: (Human) 8061, (Mouse) 14283, (Rat) 25445
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
sequence-specific DNA binding
basic leucine zipper transcription factor
in utero embryonic development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
chemotaxis
cellular defense response
vitellogenesis
female pregnancy
learning
positive regulation of cell proliferation
negative regulation of cell proliferation
response to mechanical stimulus
response to virus
response to gravity
cellular response to extracellular stimulus
response to progesterone
response to cytokine
response to drug
response to hydrogen peroxide
positive regulation of apoptotic process
positive regulation of cell cycle
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
response to corticosterone
response to cAMP
placenta blood vessel development
pri-miRNA transcription from RNA polymerase II promoter
positive regulation of DNA-templated transcription, initiation
regulation of transcription, DNA-templated
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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