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G-CSF Antibody (500-P69BT-25UG) in ELISA
Sandwich ELISA: To detect mG-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of G-CSF Polyclonal Antibody, Biotin (Product # 500-P69BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech G-CSF Polyclonal Antibody (Product # 500-P69-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mG-CSF.
Product Details
500-P69BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine G-CSF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930711
Product Specific Information
AA Sequence of recombinant protein: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine G-CSF. Anti-Murine G-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mG-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine G-CSF (500-P69) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mG-CSF.
Western Blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P69BT-1MG will be provided as 2 x 500 µg
Target Information
G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells. G-CSF is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. G-CSF is highly conserved among species and has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors, neutrophilic granulocytes and certain carcinoma cell lines. Clinical use of G-CSF has been approved for several therapeutic applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and AIDS. Further, G-CSF has been shown to be a marker protein for different carcinomas such as bladder cancer and dysfunction of the protein has been linked to Kostmann Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSF 3; G-CSF; Granulocyte colony-stimulating factor; gsf3; MGC45931; RP23-395E10.4
Gene Aliases: Csf3; Csfg; G-CSF; MGI-IG
UniProt ID: (Mouse) P09920
Entrez Gene ID: (Mouse) 12985
immune response
positive regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of actin filament polymerization
granulocyte differentiation
positive regulation of protein binding
positive regulation of peptidyl-serine phosphorylation
positive regulation of transcription factor import into nucleus
positive regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of protein kinase B signaling
cellular response to cytokine stimulus
negative regulation of neuron death
positive regulation of actin cytoskeleton reorganization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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