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Invitrogen
G3BP1 Recombinant Rabbit Monoclonal Antibody (42H56L14)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
G3BP1 Antibody (704023) in ICC/IF
For immunofluorescence analysis, MCF7 cells were treated with Heat Shock (45 degree celsius, 45 minutes), fixed and permeabilized with 0.1% Triton X-100 for detection of endogenous G3BP1 using G3BP1 Recombinant Rabbit Monoclonal Antibody (Product # 704023, 1:100 dilution) and labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000). Panel a) shows representative cells that were stained for detection and localization of G3BP1 protein (green), Panel b) is stained for ... View More
Product Details
704023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
42H56L14
Immunogen
Protein corresponding to human G3BP1 [aa140-aa249]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2895899
Product Specific Information
The antibody is predicted to react with Mouse and Rat.
Target Information
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP
Gene Aliases: G3BP; G3BP1; HDH-VIII
UniProt ID: (Human) Q13283
Entrez Gene ID: (Human) 10146
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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