Proteintech

GAD1 Polyclonal Antibody

GAD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GAD1 Antibody (10408-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GAD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10408-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GAD1 Fusion Protein Ag0666 (1-224 aa encoded by BC002815)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MASSTPSSS ATSSNAGADP NTTNLRPTTY DTWCGVAHGC TRKLGLKICG FLQRTNSLEE KSRLVSAFKE RQSSKNLLSC ENSDRDARFR RTETDFSNLF ARDLLPAKNG EEQTVQFLLE VVDILLNYVR KTFDRSTKVL DFHHPHQLLE GMEGFNLELS DHPESLEQIL VDCRDTLKYG VRTGHPRFFN QLSTGLDIIG LAGEWLTSTA NTNMPSDMRE CWLLR (1-224 aa encoded by BC002815)

Target Information

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The delta subunit of the GABA_A receptor is necessary for the GPT2-promoted breast cancer metastasis.

Theranostics

Li N,Xu X,Liu D,Gao J,Gao Y,Wu X,Sheng H,Li Q,Mi J

Tue Mar 21 00:00:00 EDT 2023

Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling.

Cell & bioscience

Liu XY,Wang K,Deng XH,Wei YH,Guo R,Liu SF,Zhu YF,Zhong JJ,Zheng JY,Wang MD,Ye QH,He JQ,Guo KH,Zhu JR,Huang SQ,Chen ZX,Lv CS,Wen L

Sat Jun 03 00:00:00 EDT 2023

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Nature cell biology

Huang D,Wang Y,Thompson JW,Yin T,Alexander PB,Qin D,Mudgal P,Wu H,Liang Y,Tan L,Pan C,Yuan L,Wan Y,Li QJ,Wang XF

Tue Feb 01 00:00:00 EST 2022

KangPiLao decoction modulates cognitive and emotional disorders in rats with central fatigue through the GABA/Glu pathway.

Frontiers in pharmacology

Xu Y,Lian Y,Li J,Zhang Y,Liu Y,Wang X,Ma J,Li F

Tue Sep 20 00:00:00 EDT 2022

Beneficial effects of metformin supplementation in hypothalamic paraventricular nucleus and arcuate nucleus of type 2 diabetic rats.

Toxicology and applied pharmacology

Yu XJ,Chen YM,Liu XJ,Bai XJ,Liu KL,Fu LY,Gao HL,Sun TZ,Shi XL,Qi J,Li Y,Kang YM

Tue Feb 15 00:00:00 EST 2022

Intrathecally administered pizotifen alleviates neuropathic and inflammatory pain in mice by enhancing GABAergic inhibition.

Neuroscience letters

Li J,Zhang X,Xu C,Liu YX,Ge C,Zhao Z,Zhu YB,Bao HG

Fri Apr 01 00:00:00 EDT 2022

Deubiquitylase OTUD3 prevents Parkinson's disease through stabilizing iron regulatory protein 2.

Cell death & disease

Jia F,Li H,Jiao Q,Li C,Fu L,Cui C,Jiang H,Zhang L

Sat Apr 30 00:00:00 EDT 2022

Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.

Phytotherapy research : PTR

Wu L,Zhang T,Chen K,Lu C,Liu XF,Zhou JL,Huang YK,Yan H,Chen Y,Zhang CJ,Li JF,Shi SQ,Ren P,Huang X

Fri Jan 01 00:00:00 EST 2021

Maternal Nicotine Exposure Alters Hippocampal Microglia Polarization and Promotes Anti-inflammatory Signaling in Juvenile Offspring in Mice.

Frontiers in pharmacology

Zhou L,Tao X,Pang G,Mu M,Sun Q,Liu F,Hu Y,Tao H,Li B,Xu K

Sun May 30 00:00:00 EDT 2021

BNST_AV ^GABA-PVN^CRF Circuit Regulates Visceral Hypersensitivity Induced by Maternal Separation in Vgat-Cre Mice.

Frontiers in pharmacology

Huang ST,Song ZJ,Liu Y,Luo WC,Yin Q,Zhang YM

Tue Apr 06 00:00:00 EDT 2021

Enriched Environment Promotes Cognitive Function Recovery following Cerebral Ischemic Injury via Upregulating GABAergic and Glutamatergic Systems in the Contralateral Hippocampus.

Evidence-based complementary and alternative medicine : eCAM

Wang Y,Wu J,Xie H,Wang L,Wang C,Wu Y

Sat Jan 30 00:00:00 EST 2021

RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.

Science advances

Eshraghi M,Ramírez-Jarquín UN,Shahani N,Nuzzo T,De Rosa A,Swarnkar S,Galli N,Rivera O,Tsaprailis G,Scharager-Tapia C,Crynen G,Li Q,Thiolat ML,Bezard E,Usiello A,Subramaniam S

Fri May 01 00:00:00 EDT 2020

Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.

Evidence-based complementary and alternative medicine : eCAM

Zhan LH,Dong YJ,Yang K,Lei SS,Li B,Teng X,Zhou C,Luo R,Yu QX,Jin HY,Lv GY,Chen SH

Mon Sep 28 00:00:00 EDT 2020

Disinhibition of PVN-projecting GABAergic neurons in AV region in BNST participates in visceral hypersensitivity in rats.

Psychoneuroendocrinology

Song Y,Meng QX,Wu K,Hua R,Song ZJ,Song Y,Qin X,Cao JL,Zhang YM

Wed Jul 01 00:00:00 EDT 2020

Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.

Translational psychiatry

Kukharsky MS,Ninkina NN,An H,Telezhkin V,Wei W,Meritens CR,Cooper-Knock J,Nakagawa S,Hirose T,Buchman VL,Shelkovnikova TA

Thu May 28 00:00:00 EDT 2020

Epidermal growth factor treatment of female mice that express APOE4 at an age of advanced pathology mitigates behavioral and cerebrovascular dysfunction.

Heliyon

Zaldua S,Damen FC,Pisharody R,Thomas R,Fan KD,Ekkurthi GK,Scheinman SB,Alahmadi S,Marottoli FM,Alford S,Cai K,Tai LM

Fri May 01 00:00:00 EDT 2020

c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.

Acta physiologica (Oxford, England)

Wang R,Zhang W,Dong Z,Qi Y,Hultström M,Zhou X,Lai EY

Wed Feb 01 00:00:00 EST 2017

c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.

Acta physiologica (Oxford, England)

Wang R,Zhang W,Dong Z,Qi Y,Hultström M,Zhou X,Lai EY

Wed Feb 01 00:00:00 EST 2017

c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.

Acta physiologica (Oxford, England)

Wang R,Zhang W,Dong Z,Qi Y,Hultström M,Zhou X,Lai EY

Wed Feb 01 00:00:00 EST 2017

c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression.

Acta physiologica (Oxford, England)

Wang R,Zhang W,Dong Z,Qi Y,Hultström M,Zhou X,Lai EY

Wed Feb 01 00:00:00 EST 2017

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Scientific reports

Chen HH,Cheng PW,Ho WY,Lu PJ,Lai CC,Tseng YM,Fang HC,Sun GC,Hsiao M,Liu CP,Tseng CJ

Mon Dec 05 00:00:00 EST 2016

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Scientific reports

Chen HH,Cheng PW,Ho WY,Lu PJ,Lai CC,Tseng YM,Fang HC,Sun GC,Hsiao M,Liu CP,Tseng CJ

Mon Dec 05 00:00:00 EST 2016

Bioinformatics

Protein Aliases: 67 kDa glutamic acid decarboxylase; FLJ45882; GAD 67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase 1; OTTHUMP00000041055

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Gene Aliases: CPSQ1; EP10; GAD; Gad-1; GAD1; GAD25; GAD44; GAD67; SCP

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UniProt ID: (Human) Q99259, (Mouse) P48318, (Rat) P18088

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Entrez Gene ID: (Human) 2571, (Mouse) 14415, (Rat) 24379

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Function(s)

glutamate decarboxylase activity protein binding glutamate binding pyridoxal phosphate binding protein heterodimerization activity protein N-terminus binding catalytic activity lyase activity carboxy-lyase activity decarboxylase

Process(es)

glutamate catabolic process glutamate decarboxylation to succinate chemical synaptic transmission neurotransmitter secretion gamma-aminobutyric acid biosynthetic process protein-pyridoxal-5-phosphate linkage neurotransmitter biosynthetic process response to drug carboxylic acid metabolic process social behavior locomotory exploration behavior

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAD1 Polyclonal Antibody

GAD1 Antibody (10408-1-AP) in IHC (P)

Product Details

10408-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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