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Invitrogen
GAD65/GAD67 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
GAD65/GAD67 Antibody (PA1-84572) in WB
Western blot was performed using Anti-GAD65/GAD67 Polyclonal Antibody (Product # PA1-84572) and a 60 kDa band corresponding to GAD65/GAD67 was observed across tested samples. Whole cell extracts (40 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probe... View More
Product Details
PA1-84572
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence DFLIEEIERLGQDL from the C-terminus of GAD.
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2263114
Product Specific Information
A suggested positive control for immunohistochemical applications is brain. In Western blot, a doublet at ~65/67 kDa has been observed.
Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa glutamic acid decarboxylase; 67 kDa glutamic acid decarboxylase; EC 4.1.1.15; FLJ45882; GAD 65; GAD 67; GAD-65; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65
Gene Aliases: 6330404F12Rik; CPSQ1; EP10; GAD; GAD(65); Gad-1; Gad-2; GAD1; GAD2; GAD25; GAD44; GAD65; GAD67; SCP
UniProt ID: (Human) Q99259, (Cat) P14748, (Human) Q05329, (Mouse) P48318, (Rat) P18088, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Human) 2571, (Cat) 493699, (Human) 2572, (Mouse) 14415, (Rat) 24379, (Mouse) 14417, (Rat) 24380
glutamate catabolic process
glutamate decarboxylation to succinate
chemical synaptic transmission
neurotransmitter secretion
gamma-aminobutyric acid biosynthetic process
protein-pyridoxal-5-phosphate linkage
neurotransmitter biosynthetic process
response to drug
carboxylic acid metabolic process
social behavior
locomotory exploration behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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