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GAD67 Antibody (PA5-94936) in ICC/IF
Immunocytochemistry analysis of GAD67 in A431 cells. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GAD67 polyclonal antibody (Product # PA5-94936) at a 3 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and developed with Strepavidin-Biotin-Complex/DAB.
Product Details
PA5-94936
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GAD67 (529-543aa PQRREKLHKVAPKIK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806742
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 67 kDa glutamic acid decarboxylase; FLJ45882; GAD 67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase 1; OTTHUMP00000041055
Gene Aliases: CPSQ1; EP10; GAD; Gad-1; GAD1; GAD25; GAD44; GAD67; SCP
UniProt ID: (Human) Q99259, (Mouse) P48318, (Rat) P18088
Entrez Gene ID: (Human) 2571, (Mouse) 14415, (Rat) 24379
glutamate catabolic process
glutamate decarboxylation to succinate
chemical synaptic transmission
neurotransmitter secretion
gamma-aminobutyric acid biosynthetic process
protein-pyridoxal-5-phosphate linkage
neurotransmitter biosynthetic process
response to drug
carboxylic acid metabolic process
social behavior
locomotory exploration behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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