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GAL4 Antibody (33-8600) in ICC/IF
Immunofluorescent analysis of Gal4 (green) in Gal4 DNA Binding Domain expressing 293T cells (Product # K1091A). The cells were fixed with formalin for 20 minutes, permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Gal4 mouse monoclonal antibody (Product # 33-8600), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a di... View More
Product Details
33-8600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Immunogen
A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533143
Product Specific Information
33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.
Target Information
GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Regulatory protein GAL4
Gene Aliases: GAL4; GAL81; YPL248C
UniProt ID: (Baker's yeast) P04386
Entrez Gene ID: (Baker's yeast) 855828
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
zinc ion binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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