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GAP43 Antibody (PA5-27448) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GAP43 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP43 Polyclonal Antibody (Product # PA5-27448) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-27448
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 238 of Human GAP43
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544924
Product Specific Information
Recommended positive controls: IMR32, mouse hippocampus, rat hippocampus.
Predicted reactivity: Cat (83%), Chimpanzee (100%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; neuron protein F1; pp46; Protein F1
Gene Aliases: B-50; Basp2; GAP-43; GAP43; PP46
UniProt ID: (Human) P17677, (Rat) P07936, (Mouse) P06837
Entrez Gene ID: (Human) 2596, (Rat) 29423, (Mouse) 14432
nervous system development
axon guidance
glial cell differentiation
axon choice point recognition
regulation of growth
tissue regeneration
cell fate commitment
regulation of filopodium assembly
axon development
multicellular organism development
cell differentiation
protein kinase C-activating G-protein coupled receptor signaling pathway
response to wounding
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