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Invitrogen
GAP43 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
GAP43 Antibody (PA5-85949) in ICC/IF
Immunofluorescence analysis of GAP43 was performed using 70% confluent log phase SH-SY5Yneuronal differentiated and undifferentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GAP43 Polyclonal Antibody (Product # PA5-85949) at 1:200 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary... View More
Product Details
PA5-85949
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within mouse GAP43 aa 178-227
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% Glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802750
Target Information
GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; neuron protein F1; pp46; Protein F1
Gene Aliases: B-50; Basp2; GAP-43; GAP43; PP46
UniProt ID: (Human) P17677, (Rat) P07936, (Mouse) P06837
Entrez Gene ID: (Human) 2596, (Rat) 29423, (Mouse) 14432
nervous system development
axon guidance
glial cell differentiation
axon choice point recognition
regulation of growth
tissue regeneration
cell fate commitment
regulation of filopodium assembly
axon development
multicellular organism development
cell differentiation
protein kinase C-activating G-protein coupled receptor signaling pathway
response to wounding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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