Proteintech

GARS Polyclonal Antibody

GARS Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

GARS Antibody (15831-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

GARS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GARS Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15831-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GARS Fusion Protein Ag8322 (390-739 aa encoded by BC007755)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MRLGDAVEQ GVINNTVLGY FIGRIYLYLT KVGISPDKLR FRQHMENEMA HYACDCWDAE SKTSYGWIEI VGCADRSCYD LSCHARATKV PLVAEKPLKE PKTVNVVQFE PSKGAIGKAY KKDAKLVMEY LAICDECYIT EMEMLLNEKG EFTIETEGKT FQLTKDMINV KRFQKTLYVE EVVPNVIEPS FGLGRIMYTV FEHTFHVREG DEQRTFFSFP AVVAPFKCSV LPLSQNQEFM PFVKELSEAL TRHGVSHKVD DSSGSIGRRY ARTDEIGVAF GVTIDFDTVN KTPHTATLRD RDSMRQIRAE ISELPSIVQD LANGNITWAD VEARYPLFEG QETGKKETIE E (390-739 aa encoded by BC007755)

Target Information

GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cell

Cui Q,Bi H,Lv Z,Wu Q,Hua J,Gu B,Huo C,Tang M,Chen Y,Chen C,Chen S,Zhang X,Wu Z,Lao Z,Sheng N,Shen C,Zhang Y,Wu ZY,Jin Z,Yang P,Liu H,Li J,Bai G

Thu Feb 16 00:00:00 EST 2023

Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes.

Nature communications

Li L,Jiang D,Liu H,Guo C,Zhao R,Zhang Q,Xu C,Qin Z,Feng J,Liu Y,Wang H,Chen W,Zhang X,Li B,Bai L,Tian S,Tan S,Yu Z,Chen L,Huang J,Zhao JY,Hou Y,Ding C

Wed Mar 29 00:00:00 EDT 2023

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Journal of hematology & oncology

Xu N,Yao Z,Shang G,Ye D,Wang H,Zhang H,Qu Y,Xu F,Wang Y,Qin Z,Zhu J,Zhang F,Feng J,Tian S,Liu Y,Zhao J,Hou J,Guo J,Hou Y,Ding C

Fri Jun 03 00:00:00 EDT 2022

Immunoprecipation Assay to Quantify the Amount of tRNAs associated with Their Interacting Proteins in Tissue and Cell Culture.

Bio-protocol

Das S,Zuko A,Thompson R,Storkebaum E,Ignatova Z

Sun Feb 20 00:00:00 EST 2022

SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Aging cell

Zhao Y,Xie L,Shen C,Qi Q,Qin Y,Xing J,Zhou D,Qi Y,Yan Z,Lin X,Dai R,Lin J,Yu W

Tue Jun 01 00:00:00 EDT 2021

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

EMBO reports

Lu X,Huang J,Wu S,Zheng Q,Liu P,Feng H,Su X,Fu H,Xi Q,Wang G

Thu Dec 03 00:00:00 EST 2020

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

EMBO reports

Lu X,Huang J,Wu S,Zheng Q,Liu P,Feng H,Su X,Fu H,Xi Q,Wang G

Thu Dec 03 00:00:00 EST 2020

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Brain : a journal of neurology

Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L

Thu Mar 01 00:00:00 EST 2018

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Human molecular genetics

Boczonadi V,Meyer K,Gonczarowska-Jorge H,Griffin H,Roos A,Bartsakoulia M,Bansagi B,Ricci G,Palinkas F,Zahedi RP,Bruni F,Kaspar B,Lochmüller H,Boycott KM,Müller JS,Horvath R

Fri Jun 15 00:00:00 EDT 2018

Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.

Nature communications

Niehues S,Bussmann J,Steffes G,Erdmann I,Köhrer C,Sun L,Wagner M,Schäfer K,Wang G,Koerdt SN,Stum M,Jaiswal S,RajBhandary UL,Thomas U,Aberle H,Burgess RW,Yang XL,Dieterich D,Storkebaum E

Fri Jul 03 00:00:00 EDT 2015

Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.

Nature communications

Niehues S,Bussmann J,Steffes G,Erdmann I,Köhrer C,Sun L,Wagner M,Schäfer K,Wang G,Koerdt SN,Stum M,Jaiswal S,RajBhandary UL,Thomas U,Aberle H,Burgess RW,Yang XL,Dieterich D,Storkebaum E

Fri Jul 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 6.1.1.14; AP-4-A synthetase; Ap4A synthetase; Charcot-Marie-Tooth neuropathy 2D; Charcot-Marie-Tooth neuropathy, neuronal type, D; Diadenosine tetraphosphate synthetase; EC 2.1.2.29; GARTF; glycine tRNA ligase; Glycine--tRNA ligase; Glycyl-tRNA synthetase; Glycyl-tRNA synthetase 1; GlyRS; MGC47764; PAIS; PGFT; PRGS; storage granule protein 23

View more View less

Gene Aliases: CMT2D; DSMAV; GARS; GARS1; Gena201; GENA202; GlyRS; HMN5; Nmf249; RGD1559871; Sgrp23; SMAD1

View more View less

UniProt ID: (Human) P41250, (Rat) Q5I0G4, (Mouse) Q9CZD3

View more View less

Entrez Gene ID: (Human) 2617, (Rat) 297113, (Mouse) 353172

View more View less

Function(s)

bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity glycine-tRNA ligase activity ATP binding protein dimerization activity nucleotide binding aminoacyl-tRNA ligase activity protein binding ligase activity aminoacyl-tRNA synthetase translational protein

Process(es)

tRNA aminoacylation for protein translation glycyl-tRNA aminoacylation diadenosine tetraphosphate biosynthetic process translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GARS Polyclonal Antibody

GARS Antibody (15831-1-AP) in ICC/IF

Product Details

15831-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!