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GATA3 Recombinant Rabbit Monoclonal Antibody (ZR358), RAbMono™

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Datasheet
Protocols
Questions & Answers

Product Details

Z2742RT

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
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Miscellaneous PubMed (Misc)

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View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ZR358

Immunogen

Recombinant full-length human GATA-3 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A

Storage buffer

tris with BSA, NP-40

Contains

<0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

This product is diluted and in a ready-to-use formulation.

A recommended positive control tissue for this product is breast or urothelial carcinoma, however positive controls are not limited to this tissue type.

The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.

Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.

Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GATA binding protein-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

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Gene Aliases: GATA3; HDR; HDRS

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UniProt ID: (Human) P23771

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Entrez Gene ID: (Human) 2625

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate determination neuron migration type IV hypersensitivity kidney development mesonephros development lens development in camera-type eye pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling transcription from RNA polymerase II promoter defense response humoral immune response signal transduction axon guidance heart development blood coagulation negative regulation of cell proliferation male gonad development response to virus anatomical structure morphogenesis post-embryonic development organ morphogenesis positive regulation of signal transduction response to gamma radiation positive regulation of endothelial cell migration regulation of neuron projection development phosphatidylinositol 3-kinase signaling erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-4 production negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process inner ear morphogenesis response to drug regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of fat cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development digestive tract development developmental growth anatomical structure formation involved in morphogenesis negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of histone H3-K14 acetylation otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration negative regulation of DNA demethylation regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of histone H3-K9 acetylation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation
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