Invitrogen

GATA4 Polyclonal Antibody

3 Published Figures

7 References

View all (29) GATA4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (13)
Published Figures (3)

GATA4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

GATA4 Antibody (PA1-102) in ICC/IF

Immunofluorescent analysis of GATA4 (green) in embryoid body endoderm generated from Gibco ® Human Episomal iPSC Line grown on Geltrex® in Essential 8TM Medium. After 2 weeks in culture, EB were dissociated with TrypLETM and re-plated onto Geltrex®-coated multi-well plates. Cells were fixed, permeabilized and blocked for immunostaining. Cells were stained with a GATA4 polyclonal antibody (Product # PA1-102) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with Alexa Fluor® 488 donkey anti rabbit ant... View More

GATA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-102

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Pooled peptides: RKEGIQTRKRKPKNLNKSK & SKQDSWNSLVLADSHGDIITA

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539875

Product Specific Information

PA1-102 has been successfully used in Western blot, FACS, immunohistochemistry (paraffin) and immunofluorescence applications with human and mouse samples. PA1-102 can be used for immunofluorescence analysis of GATA4 in the endoderm derived from human iPSCs.

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amniotic stromal stem cell-loaded hydrogel repairs cardiac tissue in infarcted rat hearts via paracrine mediators.

Journal of tissue engineering and regenerative medicine

Khan K,Makhoul G,Yu B,Jalani G,Derish I,Rutman AK,Cerruti M,Schwertani A,Cecere R

PA1-102 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the cardioprotective potential of a composite inserting human amniotic stromal mesenchymal stem cells (ASMCs) in a chitosan and hyaluronic acid (C/HA) based hydrogel in a rat MI model.

Tue Feb 01 00:00:00 EST 2022

Androgen Receptor, Although Not a Specific Marker For, Is a Novel Target to Suppress Glioma Stem Cells as a Therapeutic Strategy for Glioblastoma.

Frontiers in oncology

Zhao N,Wang F,Ahmed S,Liu K,Zhang C,Cathcart SJ,DiMaio DJ,Punsoni M,Guan B,Zhou P,Wang S,Batra SK,Bronich T,Hei TK,Lin C,Zhang C

Published figure using GATA4 polyclonal antibody (Product # PA1-102) in Western Blot

Tue Jun 08 00:00:00 EDT 2021

Neuregulin-1, in a Conducive Milieu with Wnt/BMP/Retinoic Acid, Prolongs the Epicardial-Mediated Cardiac Regeneration Capacity of Neonatal Heart Explants.

Journal of stem cells & regenerative medicine

Arora H,Lavin AC,Balkan W,Hare JM,White IA

PA1-102 was used in Immunohistochemistry to demonstrate that continuous administration of Neuregulin and retinoic acid, and brief exposure to Wnt3a and BMP-4, induced epicardial activation and subsequent recellularization in explanted, injured neonatal hearts.

Fri Aug 27 00:00:00 EDT 2021

Human Cardiac Progenitor Cells Enhance Exosome Release and Promote Angiogenesis Under Physoxia.

Frontiers in cell and developmental biology

Dougherty JA,Patel N,Kumar N,Rao SG,Angelos MG,Singh H,Cai C,Khan M

Published figure using GATA4 polyclonal antibody (Product # PA1-102) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for "Clinical Trials in a Dish".

Frontiers in bioengineering and biotechnology

Kumar N,Sridharan D,Palaniappan A,Dougherty JA,Czirok A,Isai DG,Mergaye M,Angelos MG,Powell HM,Khan M

PA1-102 was used in Immunocytochemistry to demonstrate that an aligned, coaxial 3D cardiac patch can be used for culturing of hiPSC-CMs.

Tue Oct 13 00:00:00 EDT 2020

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for "Clinical Trials in a Dish".

Frontiers in bioengineering and biotechnology

Kumar N,Sridharan D,Palaniappan A,Dougherty JA,Czirok A,Isai DG,Mergaye M,Angelos MG,Powell HM,Khan M

PA1-102 was used in Immunocytochemistry to demonstrate that an aligned, coaxial 3D cardiac patch can be used for culturing of hiPSC-CMs.

Tue Oct 13 00:00:00 EDT 2020

Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming.

Scientific reports

Zhang Z,Zhang W,Nam YJ

PA1-102 was used in Western Blotting to demonstrate the stoichiometric requirement of GHMT expression for structural and functional progresses of cardiomyocyte reprogramming.

Fri Oct 18 00:00:00 EDT 2019

Generation and characterization of a human iPSC line from an ALS patient carrying the Q66K-MATR3 mutation.

Stem cell research

Pollini D,Loffredo R,Cardano M,Conti L,Lattante S,Notarangelo A,Sabatelli M,Provenzani A

PA1-102 was used in Immunocytochemistry-immunoflourescence to generate the Q66K#44DRM induced pluripotent stem cell (iPSC) line that showed regular karyotype, expressed pluripotency-associated markers and were able to properly differentiate into the three germ layers.

Sat Dec 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4

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Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369

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Entrez Gene ID: (Human) 2626, (Mouse) 14463

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding metal ion binding DNA-binding transcription factor

Process(es)

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis atrioventricular valve formation mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis heart development regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation atrioventricular canal development response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development cardiac muscle cell differentiation Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development

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GATA4 Polyclonal Antibody

GATA4 Antibody (PA1-102) in ICC/IF

Product Details

PA1-102

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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