Invitrogen

GATA4 Monoclonal Antibody (6H10)

3 Published Figures

8 References

View all (29) GATA4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (3)

FIGURE: 1 / 5

GATA4 Antibody (MA5-15532) in WB

Western blot analysis of GATA4 using GATA4 monoclonal antibody (Product # MA5-15532) in rat fetal heart (1) and adult heart (2) tissues lysate.

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Product Details

MA5-15532

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

1:10,000

ChIP assay (ChIP)

1-3 µL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6H10

Immunogen

Purified recombinant fragment of human GATA4 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10989032

Product Specific Information

MA5-15532 targets GATA4 in indirect ELISA, WB and ChIP applications and shows reactivity with Human and mouse samples.

The MA5-15532 immunogen is purified recombinant fragment of human GATA4 expressed in E. Coli.

MA5-15532 detects GATA4 which has a predicted molecular weight of approximately 46kDa.

Target Information

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.

Cell reports. Medicine

Zhao R,Cao L,Gu WJ,Li L,Chen ZZ,Xiang J,Zhou ZY,Xu B,Zang WD,Zhou XY,Cao J,Sun K,Zhao JY

MA5-15532 was used in Chromatin immunoprecipitation to find that PA promotes methionyl-tRNA synthetase (MARS) expression and protein lysine homocysteinylation (K-Hcy) of GATA4 and results in GATA4 inhibition and abnormal heart development.

Tue Mar 21 00:00:00 EDT 2023

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.

Cell reports. Medicine

Zhao R,Cao L,Gu WJ,Li L,Chen ZZ,Xiang J,Zhou ZY,Xu B,Zang WD,Zhou XY,Cao J,Sun K,Zhao JY

Tue Mar 21 00:00:00 EDT 2023

A common variant in 11q23.3 associated with hyperlipidemia is mediated by the binding and regulation of GATA4.

NPJ genomic medicine

Chou WC,Chen WT,Shen CY

MA5-15532 was used in Western Blot, ChIP assay to suggest elevating GATA4 activity could provide a therapeutic potential for treating the development of hyperlipidemia.

Wed Jan 19 00:00:00 EST 2022

A common variant in 11q23.3 associated with hyperlipidemia is mediated by the binding and regulation of GATA4.

NPJ genomic medicine

Chou WC,Chen WT,Shen CY

MA5-15532 was used in Western Blot, ChIP assay to suggest elevating GATA4 activity could provide a therapeutic potential for treating the development of hyperlipidemia.

Wed Jan 19 00:00:00 EST 2022

Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells.

Nature communications

Low BSJ,Lim CS,Ding SSL,Tan YS,Ng NHJ,Krishnan VG,Ang SF,Neo CWY,Verma CS,Hoon S,Lim SC,Tai ES,Teo AKK

MA5-15532 was used in Flow Cytometry to reveal the importance of HNF1A in regulating GLUT2 and several genes involved in insulin secretion that can account for the insulin secretory defect clinically observed in MODY3 patients.

Tue May 25 00:00:00 EDT 2021

Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells.

Nature communications

Low BSJ,Lim CS,Ding SSL,Tan YS,Ng NHJ,Krishnan VG,Ang SF,Neo CWY,Verma CS,Hoon S,Lim SC,Tai ES,Teo AKK

Tue May 25 00:00:00 EDT 2021

Lineage-Specific Epigenomic and Genomic Activation of Oncogene HNF4A Promotes Gastrointestinal Adenocarcinomas.

Cancer research

Pan J,Silva TC,Gull N,Yang Q,Plummer JT,Chen S,Daigo K,Hamakubo T,Gery S,Ding LW,Jiang YY,Hu S,Xu LY,Li EM,Ding Y,Klempner SJ,Gayther SA,Berman BP,Koeffler HP,Lin DC

MA5-15532 was used in Western Blotting to show that gastrointestinal adenocarcinomas (GIAC)-specific master regulatory transcription factors control HNF4A via three distal enhancers to promote GIAC cell proliferation and survival.

Wed Jul 01 00:00:00 EDT 2020

BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.

Cell death & disease

Loo LSW,Soetedjo AAP,Lau HH,Ng NHJ,Ghosh S,Nguyen L,Krishnan VG,Choi H,Roca X,Hoon S,Teo AKK

MA5-15532 was used in Immunocytochemistry-immunoflourescence to profile the expression of BCL-2 family of proteins during pancreatic specification and observed an upregulation of BCL-xL, downregulation of BAK and corresponding downregulation of cleaved CASP3 representative of apoptosis.

Mon May 18 00:00:00 EDT 2020

BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.

Cell death & disease

Loo LSW,Soetedjo AAP,Lau HH,Ng NHJ,Ghosh S,Nguyen L,Krishnan VG,Choi H,Roca X,Hoon S,Teo AKK

Mon May 18 00:00:00 EDT 2020

HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells.

iScience

Ng NHJ,Jasmen JB,Lim CS,Lau HH,Krishnan VG,Kadiwala J,Kulkarni RN,Ræder H,Vallier L,Hoon S,Teo AKK

MA5-15532 was used in Immunocytochemistry-immunoflourescence to suggest that in MODY1, liver and pancreas development is perturbed early on, contributing to altered hepatic proteins and Β cell defects in patients.

Fri Jun 28 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: DNA-binding protein GATA-GT2; GAT4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; Transcription factor GATA-4

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Gene Aliases: ASD2; Gata-4; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694, (Mouse) Q08369, (Rat) P46152

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Entrez Gene ID: (Human) 2626, (Mouse) 14463, (Rat) 54254

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding metal ion binding DNA-binding transcription factor

Process(es)

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction in utero embryonic development gastrulation with mouth forming second endoderm formation heart morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis atrioventricular valve formation mitral valve formation tricuspid valve formation ventricular cardiac muscle tissue development cardiac septum development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis heart development regulation of gene expression cardiac muscle hypertrophy in response to stress signal transduction involved in regulation of gene expression BMP signaling pathway response to retinoic acid embryonic heart tube development tube morphogenesis skeletal muscle cell differentiation atrioventricular canal development response to estrogen embryonic digestive tract morphogenesis embryonic morphogenesis cardiac muscle tissue development cardiac muscle cell differentiation Sertoli cell differentiation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway SMAD protein signal transduction lung morphogenesis lung lobe formation diaphragm morphogenesis vasculogenesis involved in coronary vascular morphogenesis cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus epithelial cell fate commitment seminiferous tubule development

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GATA4 Monoclonal Antibody (6H10)

GATA4 Antibody (MA5-15532) in WB

Product Details

MA5-15532

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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