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GATA5 Antibody (PA5-47262) in ICC/IF
Immunocytochemistry analysis of GATA5 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in GATA5 polyclonal antibody (Product # PA5-47262) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Product Details
PA5-47262
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human GATA-5 Met1-Ala397
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605675
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4, and rhGATA-6 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
GATA binding factor 5 is a transcription regulator involved in embryonic development. In addition, the protein encoded by this gene contains two GATA-type zinc fingers and is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha). This interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. GATA-5 may be involved in the establishment of cardiac smooth muscle cell diversity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GATA binding factor-5; GATA-binding factor 5; GATA-binding protein 5; Transcription factor GATA-5
Gene Aliases: bB379O24.1; GATA5; GATAS
UniProt ID: (Human) Q9BWX5
Entrez Gene ID: (Human) 140628
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
zinc ion binding
transcription regulatory region DNA binding
DNA-binding transcription factor
transcription from RNA polymerase II promoter
heart development
blood coagulation
organ morphogenesis
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
cell development
anatomical structure formation involved in morphogenesis
intestinal epithelial cell differentiation
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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