Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
GCG Antibody (H00002641-D01P) in WB
Western Blot analysis of GCG expression in transfected 293T cell line (H00002641-T01) by GCG MaxPab polyclonal antibody.
Lane 1: GCG transfected lysate(20.90 KDa).
Lane 2: Non-transfected lysate.
Lane 1: GCG transfected lysate(20.90 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00002641-D01P
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GCG (NP_002045.1, 1 a.a. approximately 180 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MKSIYFVAGL FVMLVQGSWQ RSLQDTEEKS RSFSASQADP LSDPDQMNED KRHSQGTFTS DYSKYLDSRR AQDFVQWLMN TKRNRNNIAK RHDEFERHAE GTFTSDVSSY LEGQAAKEFI AWLVKGRGRR DFPEEVAIVE ELGRRHADGS FSDEMNTILD NLAARDFINW LIQTKITDRK
Target Information
Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: glicentin-related polypeptide; GLP1/2; glucagon; glucagon-like peptide 1; glucagon-like peptide 2; glucagon-like peptide-1; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon
Gene Aliases: GCG; GLP-1; GLP1; GLP2; GRPP
UniProt ID: (Human) P01275
Entrez Gene ID: (Human) 2641, (Rat) 24952
regulation of carbohydrate metabolic process
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
protein kinase A signaling
positive regulation of peptidyl-threonine phosphorylation
regulation of lipid metabolic process
protein metabolic process
positive regulation of cAMP biosynthetic process
positive regulation of protein binding
negative regulation of appetite
positive regulation of peptidyl-serine phosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
response to starvation
negative regulation of apoptotic process
positive regulation of protein kinase activity
regulation of insulin secretion
positive regulation of histone H3-K4 methylation
positive regulation of ERK1 and ERK2 cascade
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway
signal transduction
G-protein coupled receptor signaling pathway
feeding behavior
cell proliferation
cellular response to glucagon stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support