Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
GDF3 Antibody (500-P235-1MG) in ELISA
Sandwich ELISA: To detect Human GDF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of GDF3 Polyclonal Antibody (Product # 500-P235-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech GDF3 Polyclonal Antibody, Biotin (Product # 500-P235BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GDF-3.
Product Details
500-P235-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GDF-3
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929961
Product Specific Information
AA Sequence of recombinant protein: AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDF-3. Anti-Human GDF-3-specific antibody was purified by affinity chromatography employing an immobilized Human GDF-3 matrix.
Sandwich ELISA: To detect Human GDF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GDF-3 (500-P235Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GDF-3.
Western Blot: To detect Human GDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: GDF-3; Growth/differentiation factor 3
Gene Aliases: GDF3; KFS3; MCOP7; MCOPCB6; UNQ222/PRO248
UniProt ID: (Human) Q9NR23
Entrez Gene ID: (Human) 9573
skeletal system development
eye development
in utero embryonic development
response to dietary excess
endoderm development
mesoderm development
regulation of cell fate commitment
positive regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of BMP signaling pathway
notochord development
somite rostral/caudal axis specification
growth
regulation of apoptotic process
regulation of MAPK cascade
negative regulation of epidermal cell differentiation
negative regulation of myoblast differentiation
cell development
formation of anatomical boundary
SMAD protein signal transduction
primitive streak formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support