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GDF3 Antibody (PA5-47974) in ICC/IF
Immunocytochemistry analysis of GDF3 in immersion fixed BG01V human embryonic stem cells. Samples were incubated in GDF3 polyclonal antibody (Product # PA5-47974) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse GDF-3 Ala253-Gly366
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2577057
Product Specific Information
In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, rmGDF-8, and rmGDF-9 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GDF-3; Growth/differentiation factor 3; VG-1-related protein 2
Gene Aliases: C78318; ecat9; Gdf-3; GDF3; KFS3; MCOP7; MCOPCB6; UNQ222/PRO248; Vgr-2; Vgr2
UniProt ID: (Human) Q9NR23, (Mouse) Q07104
Entrez Gene ID: (Human) 9573, (Mouse) 14562
skeletal system development
eye development
in utero embryonic development
response to dietary excess
endoderm development
mesoderm development
regulation of cell fate commitment
positive regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of BMP signaling pathway
notochord development
somite rostral/caudal axis specification
growth
regulation of apoptotic process
regulation of MAPK cascade
negative regulation of epidermal cell differentiation
negative regulation of myoblast differentiation
cell development
formation of anatomical boundary
SMAD protein signal transduction
primitive streak formation
signal transduction
BMP signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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