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GDNF Antibody (500-P81BT-25UG) in ELISA
Sandwich ELISA: To detect hGDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of GDNF Polyclonal Antibody, Biotin (Product # 500-P81BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech GDNF Polyclonal Antibody (Product # 500-P81-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDNF.
Product Details
500-P81BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GDNF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930783
Product Specific Information
AA Sequence of recombinant protein: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hGDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDNF (500-P81) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDNF.
Western Blot: To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P81BT-1MG will be provided as 2 x 500 µg
Target Information
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte-derived trophic factor; ATF; Glial cell line-derived neurotrophic factor; H-GDNF; hGDNF
Gene Aliases: ATF1; ATF2; GDNF; HFB1-GDNF; HSCR3
UniProt ID: (Human) P39905
Entrez Gene ID: (Human) 2668
MAPK cascade
metanephros development
branching involved in ureteric bud morphogenesis
neural crest cell migration
organ induction
postsynaptic membrane organization
mesenchymal to epithelial transition involved in metanephros morphogenesis
signal transduction
nervous system development
positive regulation of cell proliferation
adult locomotory behavior
regulation of gene expression
postganglionic parasympathetic fiber development
peristalsis
neuron projection development
positive regulation of monooxygenase activity
positive regulation of dopamine secretion
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mRNA stabilization
enteric nervous system development
sympathetic nervous system development
regulation of dopamine uptake involved in synaptic transmission
ureteric bud formation
regulation of morphogenesis of a branching structure
positive regulation of ureteric bud formation
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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