Invitrogen

GFAP Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (235) GFAP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

GFAP Antibody (PA5-18598) in IHC (P)

Immunohistochemistry analysis of GFAP in human brain, cortex, astrocytes. Samples were incubated with GFAP polyclonal antibody (Product # PA5-18598) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

GFAP Antibody in Immunocytochemistry (ICC/IF)

GFAP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target in tissues owing to their inherent genetic constitution. Differential basal expression of GFAP was observed across the tissues where the brain samples tested positive for GFAP using product (Product # PA5-18598) in western blot. {RE}

Product Details

PA5-18598

Applications

Tested Dilution

Publications

Western Blot (WB)

0.001-0.1 µg/mL

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DGEVIKESKQEHKD) corresponding to the C-terminus amino acids of GFAP

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10984384

Product Specific Information

This antibody is predicted to react with canine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.

Methods in molecular biology (Clifton, N.J.)

Liu L,Koo Y,Russell T,Yun Y

PA5-18598 was used in Immunocytochemistry to describe a method to construct a brain-on-a-chip with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior.

Fri Jun 24 00:00:00 EDT 2022

Beneficial contribution of induced pluripotent stem cell-progeny to Connexin 47 dynamics during demyelination-remyelination.

Glia

Mozafari S,Deboux C,Laterza C,Ehrlich M,Kuhlmann T,Martino G,Baron-Van Evercooren A

PA5-18598 was used in Immunohistochemistry to investigate the expression dynamics of the oligodendrocyte specific connexin 47 (Cx47) and to some extent that of astrocyte Cx43, and whether this dynamic could be modulated by grafted induced pluripotent stem cell (iPSC)-neural progeny.

Sat May 01 00:00:00 EDT 2021

Metabolic Profiling of Suprachiasmatic Nucleus Reveals Multifaceted Effects in an Alzheimer's Disease Mouse Model.

Journal of Alzheimer's disease : JAD

Eeza MNH,Singer R,Höfling C,Matysik J,de Groot HJM,Roβner S,Alia A

Published figure using GFAP polyclonal antibody (Product # PA5-18598) in Immunohistochemistry

Mon Sep 06 00:00:00 EDT 2021

Zika Virus Targets Glioblastoma Stem Cells through a SOX2-Integrin α_vβ₅ Axis.

Cell stem cell

Zhu Z,Mesci P,Bernatchez JA,Gimple RC,Wang X,Schafer ST,Wettersten HI,Beck S,Clark AE,Wu Q,Prager BC,Kim LJY,Dhanwani R,Sharma S,Garancher A,Weis SM,Mack SC,Negraes PD,Trujillo CA,Penalva LO,Feng J,Lan Z,Zhang R,Wessel AW,Dhawan S,Diamond MS,Chen CC,Wechsler-Reya RJ,Gage FH,Hu H,Siqueira-Neto JL,Muotri AR,Cheresh DA,Rich JN

PA5-18598 was used in Immunohistochemistry (PFA fixed) to demonstrate that ZIKV preferentially infected and killed GSCs and stem-like cells in medulloblastoma and ependymoma in a SOX2-dependent manner.

Thu Feb 06 00:00:00 EST 2020

Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure.

PloS one

Liu L,Koo Y,Russell T,Gay E,Li Y,Yun Y

PA5-18598 was used in Immunocytochemistry to develop a three-dimensional (3D) brain-on-chip platform with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior.

Fri Jul 10 00:00:00 EDT 2020

Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure.

PloS one

Liu L,Koo Y,Russell T,Gay E,Li Y,Yun Y

Fri Jul 10 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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Gene Aliases: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580

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Function(s)

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

Process(es)

response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy

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GFAP Polyclonal Antibody

GFAP Antibody (PA5-18598) in IHC (P)

Product Details

PA5-18598

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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