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GFAP Antibody (PA5-85109) in ICC/IF
GFAP Polyclonal Antibody detects GFAP protein at glia cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFAP protein stained by GFAP Polyclonal Antibody (Product # PA5-85109) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-85109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GFAP.
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792257
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (94%), Rat (94%), Pig (95%), Bovine (97%).
Positive Control: U87-MG, mouse brain, rat brain, GFAP-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein
Gene Aliases: AI836096; ALXDRD; GFAP
UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995
Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
intermediate filament-based process
intermediate filament organization
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
long-term synaptic potentiation
regulation of protein complex assembly
regulation of chaperone-mediated autophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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