Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
GFAP Antibody (MA1-35377) in ICC/IF
Immunofluorescent analysis of GFAP (green) in human diabetic Muller cells. Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature, and blocked with 5% normal goat serum for 1 hour at room temperature. Cells were probed with a GFAP monoclonal antibody (Product # MA1-35377) at a dilution of 1:25 for 1 hour at room temperature, washed with PBS, and then incubated with an Alexa Fluor 488-conjugated anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 ho... View More
Product Details
MA1-35377
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fish,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6F2
Immunogen
GFAP from human brain.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.01M sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_1074614
Product Specific Information
The antibody stains cells containing GFAP. The antibody only stains the 56kD GFAP band in immunoblots performed on crude brain extract. It reacts also with mouse GFAP
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP)
Gene Aliases: ALXDRD; GFAP
UniProt ID: (Human) E9PAX3
Entrez Gene ID: (Human) 2670
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
regulation of protein complex assembly
intermediate filament organization
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
long-term synaptic potentiation
regulation of chaperone-mediated autophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support