Product References
Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model.
PloS one
Obara K,Shirai K,Hamada Y,Arakawa N,Hasegawa A,Takaoka N,Aki R,Hoffman RM,Amoh Y
14-9892-82 was used in Immunohistochemistry and Immunocytochemistry to demonstrate future clinical potential of HAP-stem-cell repair of ICH, currently a recalcitrant disease.
Tue Jan 17 00:00:00 EST 2023
Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model.
PloS one
Obara K,Shirai K,Hamada Y,Arakawa N,Hasegawa A,Takaoka N,Aki R,Hoffman RM,Amoh Y
14-9892-82 was used in Immunohistochemistry and Immunocytochemistry to demonstrate future clinical potential of HAP-stem-cell repair of ICH, currently a recalcitrant disease.
Tue Jan 17 00:00:00 EST 2023
Apolipoprotein E ε4 disrupts oligodendrocyte differentiation by interfering with astrocyte-derived lipid transport.
Journal of neurochemistry
Mok KK,Yeung SH,Cheng GW,Ma IW,Lee RH,Herrup K,Tse KH
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry (Frozen)
Sat Apr 01 00:00:00 EDT 2023
Altered Tau Kinase Activity in rTg4510 Mice after a Single Interfaced CHIMERA Traumatic Brain Injury.
International journal of molecular sciences
Cheng WH,Cheung H,Kang A,Fan J,Cooper J,Anwer M,Barron C,Wilkinson A,Hu G,Yue J,Cripton PA,Vocadlo DJ,Wellington CL
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry (Paraffin)
Mon May 29 00:00:00 EDT 2023
Diospyros lotus leaf extract and its main component myricitrin regulate pruritus through the inhibition of astrocyte activation.
Experimental and therapeutic medicine
Shin JY,Cho BO,Park JH,Kang ES,Kim YS,Jang SI
14-9892-82 was used in Western Blotting to suggest that Diospyros lotus leaf extract and myricetin can be used as oral therapeutic agents for the treatment and management of pruritus.
Sat Jul 01 00:00:00 EDT 2023
Single-cell transcriptomics and surface epitope detection in human brain epileptic lesions identifies pro-inflammatory signaling.
Nature neuroscience
Kumar P,Lim A,Hazirah SN,Chua CJH,Ngoh A,Poh SL,Yeo TH,Lim J,Ling S,Sutamam NB,Petretto E,Low DCY,Zeng L,Tan EK,Arkachaisri T,Yeo JG,Ginhoux F,Chan D,Albani S
14-9892-82 was used in Immunohistochemistry-immunofluorescence to provide insight into the immune microenvironment in epileptic tissue, which may aid the development of new therapeutics.
Fri Jul 01 00:00:00 EDT 2022
Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.
iScience
Battistella E,Schniete J,Wesencraft K,Quintana JF,McConnell G
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Fri Sep 16 00:00:00 EDT 2022
The spatial transcriptomic landscape of non-small cell lung cancer brain metastasis.
Nature communications
Zhang Q,Abdo R,Iosef C,Kaneko T,Cecchini M,Han VK,Li SS
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Mon Oct 10 00:00:00 EDT 2022
Glial receptor PLXNB2 regulates schizophrenia-related stress perception via the amygdala.
Frontiers in immunology
Xuan FL,Yan L,Li Y,Fan F,Deng H,Gou M,Chithanathan K,Heinla I,Yuan L,Seppa K,Zharkovsky A,Kalda A,Hong LE,Hu GF,Tan Y,Tian L
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Mon Nov 07 00:00:00 EST 2022
Inhibition of Calpain Attenuates Degeneration of Substantia Nigra Neurons in the Rotenone Rat Model of Parkinson's Disease.
International journal of molecular sciences
Zaman V,Drasites KP,Myatich A,Shams R,Shields DC,Matzelle D,Haque A,Banik NL
14-9892-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find that both isoforms are activated in a nigrostriatal pathway with increased phosphorylated synuclein following the administration of rotenone in Lewis rats, but calpain isoforms played different roles in neuronal survival.
Thu Nov 10 00:00:00 EST 2022
Neurovascular dysfunction in GRN-associated frontotemporal dementia identified by single-nucleus RNA sequencing of human cerebral cortex.
Nature neuroscience
Gerrits E,Giannini LAA,Brouwer N,Melhem S,Seilhean D,Le Ber I,Kamermans A,Kooij G,de Vries HE,Boddeke EWGM,Seelaar H,van Swieten JC,Eggen BJL
14-9892-82 was used in Immunohistochemistry to indicate a perturbed blood-brain barrier and suggest that the neurovascular unit is severely affected in frontotemporal dementia-progranulin gene.
Mon Aug 01 00:00:00 EDT 2022
Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain.
Nature communications
Rayaprolu S,Bitarafan S,Santiago JV,Betarbet R,Sunna S,Cheng L,Xiao H,Nelson RS,Kumar P,Bagchi P,Duong DM,Goettemoeller AM,Oláh VJ,Rowan M,Levey AI,Wood LB,Seyfried NT,Rangaraju S
14-9892-82 was used in Immunohistochemistry-immunofluorescence to describe a mouse line for cell type-specific expression of biotin ligase TurboID, for in vivo biotinylation of proteins.
Wed May 25 00:00:00 EDT 2022
Monomeric C-reactive protein induces the cellular pathology of Alzheimer's disease.
Alzheimer's & dementia (New York, N. Y.)
Gan Q,Wong A,Zhang Z,Na H,Tian H,Tao Q,Rajab IM,Potempa LA,Qiu WQ
14-9892-82 was used in Immunocytochemistry-immunoflourescence to use different primary neuronal cells and investigate if C-reactive protein induces cellular Alzheimer's disease pathology depending on apolipoprotein E genotypes.
Tue Jul 19 00:00:00 EDT 2022
Trimethylamine N-Oxide Reduces Neurite Density and Plaque Intensity in a Murine Model of Alzheimer's Disease.
Journal of Alzheimer's disease : JAD
Zarbock KR,Han JH,Singh AP,Thomas SP,Bendlin BB,Denu JM,Yu JJ,Rey FE,Ulland TK
14-9892-82 was used in Immunohistochemistry-immunofluorescence to suggest that elevated plasma TMAO impacts AD pathology via reductions in neurite density.
Tue Nov 15 00:00:00 EST 2022
Activated microglia mitigate Aβ-associated tau seeding and spreading.
The Journal of experimental medicine
Gratuze M,Chen Y,Parhizkar S,Jain N,Strickland MR,Serrano JR,Colonna M,Ulrich JD,Holtzman DM
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Mon Aug 02 00:00:00 EDT 2021
Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
Aging cell
Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ
14-9892-82 was used in Immunohistochemistry to demonstrate that the interactions of endothelial CD31 with monomeric C-reactive protein (mCRP) versus ApoE were linked with shortened neurovasculature for Alzheimer's disease (AD) pathology and cognition.
Mon Nov 01 00:00:00 EDT 2021
Regionally diverse astrocyte subtypes and their heterogeneous response to EAE.
Glia
Borggrewe M,Grit C,Vainchtein ID,Brouwer N,Wesseling EM,Laman JD,Eggen BJL,Kooistra SM,Boddeke EWGM
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Sat May 01 00:00:00 EDT 2021
microRNAs (miR 9, 124, 155 and 224) transdifferentiate mouse macrophages to neurons.
Experimental cell research
Challagundla N,Agrawal-Rajput R
14-9892-82 was used in Immunocytochemistry to report a novel approach of microRNA (miR)- induced trans-differentiation of macrophages (CD11b high) to induced neuronal cells (iNCs) (neuronal markershigh- Nestin, Nurr1, Map2, NSE, Tubb3 and Mash1) without exogenous use of transcription factors. miR 9, 124, 155 and 224 successfully transdifferentiated macrophages to neurons with transient stem cell-like phenotype.
Sat May 01 00:00:00 EDT 2021
CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.
Neurology(R) neuroimmunology & neuroinflammation
Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Mon Mar 01 00:00:00 EST 2021
CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.
Neurology(R) neuroimmunology & neuroinflammation
Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Mon Mar 01 00:00:00 EST 2021
The Role of Endoplasmic Reticulum in the Differential Endurance against Redox Stress in Cortical and Spinal Astrocytes from the Newborn SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis.
Antioxidants (Basel, Switzerland)
Marini C,Cossu V,Kumar M,Milanese M,Cortese K,Bruno S,Bellese G,Carta S,Zerbo RA,Torazza C,Bauckneht M,Venturi C,Raffa S,Orengo AM,Donegani MI,Chiola S,Ravera S,Castellani P,Morbelli S,Sambuceti G,Bonanno G
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Flow Cytometry
Mon Aug 30 00:00:00 EDT 2021
Developmental Role of Adenosine Kinase in the Cerebellum.
eNeuro
Gebril H,Wahba A,Zhou X,Lai T,Alharfoush E,DiCicco-Bloom E,Boison D
14-9892-82 was used in Immunohistochemistry to demonstrate an association between neuronal ADK expression and developmental processes of the CB, which supports a functional role of ADK-L in the plasticity of the CB.
Fri Jul 02 00:00:00 EDT 2021
Developmental Role of Adenosine Kinase in the Cerebellum.
eNeuro
Gebril H,Wahba A,Zhou X,Lai T,Alharfoush E,DiCicco-Bloom E,Boison D
14-9892-82 was used in Immunohistochemistry to demonstrate an association between neuronal ADK expression and developmental processes of the CB, which supports a functional role of ADK-L in the plasticity of the CB.
Fri Jul 02 00:00:00 EDT 2021
Studies of involvement of G-protein coupled receptor-3 in cannabidiol effects on inflammatory responses of mouse primary astrocytes and microglia.
PloS one
Wu J,Chen N,Liu Y,Godlewski G,Kaplan HJ,Shrader SH,Song ZH,Shao H
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Thu Oct 21 00:00:00 EDT 2021
Bidens pilosa Extract Administered after Symptom Onset Attenuates Glial Activation, Improves Motor Performance, and Prolongs Survival in a Mouse Model of Amyotrophic Lateral Sclerosis.
Oxidative medicine and cellular longevity
Kosuge Y,Kaneko E,Nango H,Miyagishi H,Ishige K,Ito Y
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunocytochemistry
Thu Sep 17 00:00:00 EDT 2020
Neuronal Mitochondria Modulation of LPS-Induced Neuroinflammation.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Harland M,Torres S,Liu J,Wang X
14-9892 was used in Western Blotting to suggest that neuronal mitofusin2 contributes to the regulation of neuroinflammation.
Wed Feb 19 00:00:00 EST 2020
Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.
Neural regeneration research
Oh JY,Hwang TY,Jang JH,Park JY,Ryu Y,Lee H,Park HJ
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry (Frozen)
Sun Nov 01 00:00:00 EDT 2020
Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.
Neural regeneration research
Oh JY,Hwang TY,Jang JH,Park JY,Ryu Y,Lee H,Park HJ
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry (Frozen)
Sun Nov 01 00:00:00 EDT 2020
Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology.
Frontiers in psychiatry
Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV
14-9892 was used in Western Blotting to study exosomal amyloid and tau protein levels in people with schizophrenia.
Sat Mar 27 00:00:00 EDT 2021
Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology.
Frontiers in psychiatry
Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV
14-9892 was used in Western Blotting to study exosomal amyloid and tau protein levels in people with schizophrenia.
Sat Mar 27 00:00:00 EDT 2021
Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease.
Molecular neurodegeneration
Rayaprolu S,Gao T,Xiao H,Ramesha S,Weinstock LD,Shah J,Duong DM,Dammer EB,Webster JA,Lah JJ,Wood LB,Betarbet R,Levey AI,Seyfried NT,Rangaraju S
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Thu May 07 00:00:00 EDT 2020
Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease.
Molecular neurodegeneration
Rayaprolu S,Gao T,Xiao H,Ramesha S,Weinstock LD,Shah J,Duong DM,Dammer EB,Webster JA,Lah JJ,Wood LB,Betarbet R,Levey AI,Seyfried NT,Rangaraju S
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Thu May 07 00:00:00 EDT 2020
Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.
Frontiers in pharmacology
Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunocytochemistry
Mon Sep 28 00:00:00 EDT 2020
Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.
Frontiers in pharmacology
Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunocytochemistry
Mon Sep 28 00:00:00 EDT 2020
Oxidative stress in the retina and retinal pigment epithelium (RPE): Role of aging, and DJ-1.
Redox biology
Upadhyay M,Milliner C,Bell BA,Bonilha VL
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Thu Oct 01 00:00:00 EDT 2020
Neuroprotective effect of ACTH on collagenase-induced peri-intraventricular hemorrhage in newborn male rats.
Scientific reports
Martins CA,Neves LT,de Oliveira MMBP,Bagatini PB,Barboza R,Mestriner RG,Xavier LL,Rasia-Filho AA
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunocytochemistry
Tue Oct 20 00:00:00 EDT 2020
Neuroprotective effect of ACTH on collagenase-induced peri-intraventricular hemorrhage in newborn male rats.
Scientific reports
Martins CA,Neves LT,de Oliveira MMBP,Bagatini PB,Barboza R,Mestriner RG,Xavier LL,Rasia-Filho AA
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunocytochemistry
Tue Oct 20 00:00:00 EDT 2020
Obesity-Induced Cellular Senescence Drives Anxiety and Impairs Neurogenesis.
Cell metabolism
Ogrodnik M,Zhu Y,Langhi LGP,Tchkonia T,Krüger P,Fielder E,Victorelli S,Ruswhandi RA,Giorgadze N,Pirtskhalava T,Podgorni O,Enikolopov G,Johnson KO,Xu M,Inman C,Palmer AK,Schafer M,Weigl M,Ikeno Y,Burns TC,Passos JF,von Zglinicki T,Kirkland JL,Jurk D
14-9892-82 was used in Flow Cytometry to provide proof-of-concept evidence that senescent cells are major contributors to obesity-induced anxiety and that senolytics are a potential new therapeutic avenue for treating neuropsychiatric disorders.
Tue May 07 00:00:00 EDT 2019
FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation.
Nature communications
Yan J,Zhao Q,Gabrusiewicz K,Kong LY,Xia X,Wang J,Ott M,Xu J,Davis RE,Huo L,Rao G,Sun SC,Watowich SS,Heimberger AB,Li S
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunofluorescence
Fri Jan 25 00:00:00 EST 2019
CHIMERA repetitive mild traumatic brain injury induces chronic behavioural and neuropathological phenotypes in wild-type and APP/PS1 mice.
Alzheimer's research & therapy
Cheng WH,Martens KM,Bashir A,Cheung H,Stukas S,Gibbs E,Namjoshi DR,Button EB,Wilkinson A,Barron CJ,Cashman NR,Cripton PA,Wellington CL
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunohistochemistry
Sat Jan 12 00:00:00 EST 2019
Characterisation of the inflammatory cytokine and growth factor profile in a rabbit model of proliferative vitreoretinopathy.
Scientific reports
Wong CW,Cheung N,Ho C,Barathi V,Storm G,Wong TT
14-9892 was used in Immunohistochemistry to demonstrate that inflammatory cytokines IL-6, -8, elevation post proliferative vitreoretinopathy (PVR) induction is followed by elevated levels of fibroproliferative growth factors, Ang2, PlGF,VEGF and PDGF-BB in the development of PVR, which will guide future studies targeting appropriate therapeutic strategies for the treatment of PVR.
Mon Oct 28 00:00:00 EDT 2019
Adverse early life environment induces anxiety-like behavior and increases expression of FKBP5 mRNA splice variants in mouse brain.
Physiological genomics
Ke X,Fu Q,Majnik A,Cohen S,Liu Q,Lane R
14-9892-82 was used in Immunohistochemistry to investigate if adverse early life environment will increase expression of FK506-binding protein 5 and its mRNA splice variants in prefrontal cortex, hippocampus, and hypothalamus as well as increase anxiety in adult mice.
Thu Nov 01 00:00:00 EDT 2018
Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning.
Nature communications
Weider M,Starost LJ,Groll K,Küspert M,Sock E,Wedel M,Fröb F,Schmitt C,Baroti T,Hartwig AC,Hillgärtner S,Piefke S,Fadler T,Ehrlich M,Ehlert C,Stehling M,Albrecht S,Jabali A,Schöler HR,Winkler J,Kuhlmann T,Wegner M
14-9892 was used in Immunohistochemistry-immunofluorescence to identify Nfat proteins as Sox10 targets and regulators of oligodendroglial differentiation in rodents and humans.
Fri Mar 02 00:00:00 EST 2018
Depletion of microglia exacerbates postischemic inflammation and brain injury.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Flow Cytometry
Thu Jun 01 00:00:00 EDT 2017
Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats.
Frontiers in molecular neuroscience
Wu LY,Ye ZN,Zhou CH,Wang CX,Xie GB,Zhang XS,Gao YY,Zhang ZH,Zhou ML,Zhuang Z,Liu JP,Hang CH,Shi JX
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Immunofluorescence
Thu Oct 01 00:00:00 EDT 2020
Vitamin D receptor expression is essential during retinal vascular development and attenuation of neovascularization by 1, 25(OH)2D3.
PloS one
Jamali N,Wang S,Darjatmoko SR,Sorenson CM,Sheibani N
14-9892 was used in Immunohistochemistry to identify that vitamin D receptor expression plays a significant role during retinal vascular development, especially during maturation of retinal vasculature.
Tue Jan 16 00:00:00 EST 2018
Cellular Microbiology of Mycoplasma canis.
Infection and immunity
Michaels DL,Leibowitz JA,Azaiza MT,Shil PK,Shama SM,Kutish GF,Distelhorst SL,Balish MF,May MA,Brown DR
14-9892 was used in Functional assays to elucidate that M. canis has the capacity to influence meningoencephalitis through interactions within the multicellular and neurochemical in vivo milieu.
Wed Jun 01 00:00:00 EDT 2016
Estrogen receptor-α is localized to neurofibrillary tangles in Alzheimer's disease.
Scientific reports
Wang C,Zhang F,Jiang S,Siedlak SL,Shen L,Perry G,Wang X,Tang B,Zhu X
14989282 was used in immunohistochemistry - paraffin section to discuss the role of the estrogen receptor in Alzheimer disease pathogenesis
Wed Feb 03 00:00:00 EST 2016
Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.
PloS one
Vazquez-Cintron EJ,Vakulenko M,Band PA,Stanker LH,Johnson EA,Ichtchenko K
Published figure using GFAP monoclonal antibody (Product # 14-9892-82) in Flow Cytometry
Wed Dec 03 00:00:00 EST 2014
