Invitrogen

GFAP Monoclonal Antibody (S.880.0)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (235) GFAP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 5

GFAP Antibody (MA5-15086) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse Liver (Lane 3) and Mouse Ovary (Lane 4). The blot was probed with Anti-GFAP Monoclonal Antibody (S.880.0) (Product # MA5-15086, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 50 kDa band corresponding to GFAP was observed in the Mouse and Rat brain, while it was not detected in Mouse Liver... View More

GFAP Antibody in Immunohistochemistry (IHC)

GFAP Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target in tissues owing to their inherent genetic constitution. Differential basal expression of GFAP was observed across the tissues where the brain samples tested positive for GFAP using product (Product # MA5-15086) in western blot. {RE}

Product Details

MA5-15086

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S.880.0

Immunogen

Native GFAP purified from pig spinal cord

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

231 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10981734

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

MA5-15086 was used in Western Blotting to investigate whether microbiome depletion induced by administration of an antibiotic cocktail (ABX) affects spleen and brain function.

Wed Feb 01 00:00:00 EST 2023

Mature iPSC-derived astrocytes of an ALS/FTD patient carrying the TDP43 mutation display a mild reactive state and release polyP toxic to motoneurons.

Frontiers in cell and developmental biology

Rojas F,Aguilar R,Almeida S,Fritz E,Corvalán D,Ampuero E,Abarzúa S,Garcés P,Amaro A,Diaz I,Arredondo C,Cortes N,Sanchez M,Mercado C,Varela-Nallar L,Gao FB,Montecino M,van Zundert B

MA5-15086 was used in Immunocytochemistry to establish that human astrocytes carrying the TDP43A90V mutation exhibit a cell-autonomous pathological signature, hence providing an experimental model to decipher the molecular mechanisms underlying the generation of the neurotoxic phenotype.

Sat Mar 23 00:00:00 EDT 2024

Cardiac Investigations in Sudden Unexpected Death in DEPDC5-Related Epilepsy.

Annals of neurology

Bacq A,Roussel D,Bonduelle T,Zagaglia S,Maletic M,Ribierre T,Adle-Biassette H,Marchal C,Jennesson M,An I,Picard F,Navarro V,Sisodiya SM,Baulac S

MA5-15086 was used in Immunohistochemistry to demonstrate DEPDC5 haploinsufficiency predisposes to primary cardiac defects that could contribute to SUDEP and therefore impact the clinical management of patients at high risk of SUDEP.

Sat Jan 01 00:00:00 EST 2022

Cardiac Investigations in Sudden Unexpected Death in DEPDC5-Related Epilepsy.

Annals of neurology

Bacq A,Roussel D,Bonduelle T,Zagaglia S,Maletic M,Ribierre T,Adle-Biassette H,Marchal C,Jennesson M,An I,Picard F,Navarro V,Sisodiya SM,Baulac S

Sat Jan 01 00:00:00 EST 2022

Nestin contributes to laser choroidal and retinal neovascularization.

Molecular vision

Miloudi S,Valensi M,El Sanharawi M,Abitbol MM,Behar-Cohen F,Versaux-Botteri C

MA5-15086 was used in Immunohistochemistry (Paraffin), Western Blot to examine the role of nestin in choroidal and retinal neovascularization.

Thu Oct 27 00:00:00 EDT 2022

Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism.

PloS one

Smith LK,Babcock IW,Minamide LS,Shaw AE,Bamburg JR,Kuhn TB

MA5-15086 was used in Immunocytochemistry to report that the HIV viral envelope glycoprotein gp120 induces the formation of aberrant, rod-shaped cofilin-actin inclusions (rods) in cultured mouse hippocampal neurons via a signaling pathway common to other neurodegenerative stimuli including oligomeric, soluble amyloid-β and proinflammatory cytokines.

Tue Oct 12 00:00:00 EDT 2021

Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism.

PloS one

Smith LK,Babcock IW,Minamide LS,Shaw AE,Bamburg JR,Kuhn TB

Tue Oct 12 00:00:00 EDT 2021

Low-Power Sonication Can Alter Extracellular Vesicle Size and Properties.

Cells

Nizamudeen ZA,Xerri R,Parmenter C,Suain K,Markus R,Chakrabarti L,Sottile V

MA5-15086 was used in Immunohistochemistry-immunofluorescence to show that beyond the intended disaggregation effect, sonication using the lowest power setting available was enough to alter the size distribution, membrane integrity, and uptake of EVs in cultured cells.

Tue Sep 14 00:00:00 EDT 2021

Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.

Scientific reports

Higuchi A,Kao SH,Ling QD,Chen YM,Li HF,Alarfaj AA,Munusamy MA,Murugan K,Chang SC,Lee HC,Hsu ST,Kumar SS,Umezawa A

MA5-15086 was used in immunocytochemistry to examine how the elasticity of the matrix affects the pluripotency of hPSCs

Mon Dec 14 00:00:00 EST 2015

Mutant disrupted-in-schizophrenia 1 in astrocytes: focus on glutamate metabolism.

Journal of neuroscience research

Abazyan S,Yang EJ,Abazyan B,Xia M,Yang C,Rojas C,Slusher B,Sattler R,Pletnikov M

MA5-15086 was used in western blot to characterize the mutant disrupted-in-schizophrenia 1 protein in astrocytes

Mon Dec 01 00:00:00 EST 2014

Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.

Glia

Sbai O,Ould-Yahoui A,Ferhat L,Gueye Y,Bernard A,Charrat E,Mehanna A,Risso JJ,Chauvin JP,Fenouillet E,Rivera S,Khrestchatisky M

MA5-15086 was used in immunocytochemistry to study the intracellular distribution, transport, and secretion of MMP-2, MMP-9, TIMP-1, and TIMP-2 in cultured reactive astrocytes

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

View more View less

Gene Aliases: AI836096; ALXDRD; GFAP

View more View less

UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995

View more View less

Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580

View more View less

Function(s)

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

Process(es)

response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GFAP Monoclonal Antibody (S.880.0)

GFAP Antibody (MA5-15086) in WB

Product Details

MA5-15086

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!