Invitrogen

GFP Polyclonal Antibody, Biotin

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (114) GFP antibodies

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GFP Antibody (A10259) in WB

Western blot was performed using Biotin-conjugated Anti-GFP Rabbit Polyclonal Antibody (Product # A10259) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), H3-GFP, 40 µg (Lane 3), H3-GFP, 20 µg (Lane 4), H3-GFP, 10 µg (Lane 5), p65-GFP, 40 µg (Lane 6), His-p65-YFP, 40 µg (Lane 7), H3-mCherry, 40 µg (Lane 8) and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved p... View More

GFP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GFP Antibody — Antibody specificity was demonstrated by detection of different targets fused to GFP tag in transiently transfected lysates tested. Relative detection of GFP tag was observed across different proteins fused with GFP in H3-GFP (Lane 3-5) and p65-GFP (Lane 6). GFP-variant, YFP is also being detected in His-p65-YFP lysate (Lane 7), using Biotin-conjugated Anti-GFP Rabbit Polyclonal Antibody (Product # A10259) in Western Blot. {RE}

Product Details

A10259

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

View 1 publication 1 publication

GST Pull Down (GPD)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534021

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brain transplantation of genetically corrected Sanfilippo type B neural stem cells induces partial cross-correction of the disease.

Molecular therapy. Methods & clinical development

Pearse Y,Clarke D,Kan SH,Le SQ,Sanghez V,Luzzi A,Pham I,Nih LR,Cooper JD,Dickson PI,Iacovino M

Published figure using GFP polyclonal antibody (Product # A10259) in Immunohistochemistry

Thu Dec 08 00:00:00 EST 2022

Revealing protein-protein interactions at the transcriptome scale by sequencing.

Molecular cell

Johnson KL,Qi Z,Yan Z,Wen X,Nguyen TC,Zaleta-Rivera K,Chen CJ,Fan X,Sriram K,Wan X,Chen ZB,Zhong S

A10259 was used in GST Pull Down to map protein-protein interactions (PPIs) en masse by PROPER-seq (protein-protein interaction sequencing).

Thu Oct 07 00:00:00 EDT 2021

The Raphe Dopamine System Controls the Expression of Incentive Memory.

Neuron

Lin R,Liang J,Wang R,Yan T,Zhou Y,Liu Y,Feng Q,Sun F,Li Y,Li A,Gong H,Luo M

A10259 was used in Immunohistochemistry to reveal a specialized DA subsystem important for memory expression and suggests new targets for interventions against opioid addiction.

Wed May 06 00:00:00 EDT 2020

Podoplanin neutralization improves cardiac remodeling and function after acute myocardial infarction.

JCI insight

Cimini M,Garikipati VNS,de Lucia C,Cheng Z,Wang C,Truongcao MM,Lucchese AM,Roy R,Benedict C,Goukassian DA,Koch WJ,Kishore R

A10259 was used in Flow cytometry/Cell sorting to indicate that modulating the interaction between podoplanin positive cells with the immune cells after myocardial infarction positively affects immune cell recruitment and may represent a novel therapeutic target to augment post-MI cardiac repair, regeneration and function.

Tue Jul 09 00:00:00 EDT 2019

Genetically Corrected iPSC-Derived Neural Stem Cell Grafts Deliver Enzyme Replacement to Affect CNS Disease in Sanfilippo B Mice.

Molecular therapy. Methods & clinical development

Clarke D,Pearse Y,Kan SH,Le SQ,Sanghez V,Cooper JD,Dickson PI,Iacovino M

A10259 was used in Immunohistochemistry-immunofluorescence to report that, ex vivo, lentiviral correction of Naglu -/- neural stem cells derived from Naglu -/- mice corrected their lysosomal pathology and allowed them to secrete a functional NAGLU enzyme.

Fri Sep 21 00:00:00 EDT 2018

Oxytocin receptors are expressed on dopamine and glutamate neurons in the mouse ventral tegmental area that project to nucleus accumbens and other mesolimbic targets.

The Journal of comparative neurology

Peris J,MacFadyen K,Smith JA,de Kloet AD,Wang L,Krause EG

A10259 was used in immunohistochemistry - free floating to characterize oxytocin receptor-expressing neurons originating within the ventral tegmental area

Sat Apr 01 00:00:00 EDT 2017

Stem cell-derived endochondral cartilage stimulates bone healing by tissue transformation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Bahney CS,Hu DP,Taylor AJ,Ferro F,Britz HM,Hallgrimsson B,Johnstone B,Miclau T,Marcucio RS

A10259 was used in immunohistochemistry - frozen section to use a murine model of a segmental tibia defect to test the utility of bone regeneration from a cartilage graft

Tue Dec 09 00:00:00 EST 2014

Bioinformatics

Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

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GFP Polyclonal Antibody, Biotin

GFP Antibody (A10259) in WB

Product Details

A10259

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

GST Pull Down (GPD)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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