Invitrogen

GFP Polyclonal Antibody

GFP Antibody in Immunocytochemistry (ICC/IF)

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GFP Antibody (PA1-9533) in ICC/IF

Cells 2022 - Asp-GFP localization during kinetochore-driven microtubule regrowth (KDMTR) after colcemid-induced MT depolymerization. ( A ) S2 cells expressing both Asp-GFP and Cherry-tubulin were fixed and stained with anti-alpha-tubulin (red) and anti-GFP (green) antibodies and counterstained for DNA (DAPI, blue). Panel ( A1 ) shows an early KDMTR stage; panels ( A2 , A3 ) show more advanced KDMTR phases characterized ... View More

GFP Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-9533

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 7 publications 7 publications

ELISA (ELISA)

1:500-1:2,000

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant Green Fluorescent fusion Protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgY fraction

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074893

Product Specific Information

PA1-9533 detects Green Fluorescence Protein in N/A samples.

PA1-9533 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9533 detects a 32 kDa protein representing Green Fluorescence Protein.

PA1-9533 immunogen is a purified recombinant green fluorescence protein.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic Control of Kinetochore-Driven Microtubule Growth in Drosophila Mitosis.

Cells

Popova JV,Pavlova GA,Razuvaeva AV,Yarinich LA,Andreyeva EN,Anders AF,Galimova YA,Renda F,Somma MP,Pindyurin AV,Gatti M

PA1-9533 was used in Immunocytochemistry-immunofluorescence to propose that kinetochores capture the plus ends of MTs nucleated in their vicinity and that these MTs elongate at kinetochores through the action of Mast/Orbit.

Wed Jul 06 00:00:00 EDT 2022

Genetic Control of Kinetochore-Driven Microtubule Growth in Drosophila Mitosis.

Cells

Popova JV,Pavlova GA,Razuvaeva AV,Yarinich LA,Andreyeva EN,Anders AF,Galimova YA,Renda F,Somma MP,Pindyurin AV,Gatti M

Wed Jul 06 00:00:00 EDT 2022

Carboxypeptidase E Independently Changes Microtubule Glutamylation, Dendritic Branching, and Neuronal Migration.

ASN neuro

Liang C,Carrel D,Singh NK,Hiester LL,Fanget I,Kim H,Firestein BL

PA1-9533 was used in Immunocytochemistry, Western Blot to suggest that CPE changes MT glutamylation and redistribution of p150Glued and that this function of CPE is independent of its role in shaping dendrite development but plays a partial role in regulating cortical neuron migration.

Mon Jan 17 00:00:00 EST 2022

Carboxypeptidase E Independently Changes Microtubule Glutamylation, Dendritic Branching, and Neuronal Migration.

ASN neuro

Liang C,Carrel D,Singh NK,Hiester LL,Fanget I,Kim H,Firestein BL

Mon Jan 17 00:00:00 EST 2022

Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.

Molecular neurobiology

Omelchenko A,Menon H,Donofrio SG,Kumar G,Chapman HM,Roshal J,Martinez-Montes ER,Wang TL,Spaller MR,Firestein BL

PA1-9533 was used in Western Blot to investigate the effect of he cysteine-rich PDZ-binding protein, CRIPT, on dendritic branching in hippocampal neurons.

Fri May 01 00:00:00 EDT 2020

Characterization hiPSC-derived neural progenitor cells and neurons to investigate the role of NOS1AP isoforms in human neuron dendritogenesis.

Molecular and cellular neurosciences

Crosta CM,Hernandez K,Bhattiprolu AK,Fu AY,Moore JC,Clarke SG,Dudzinski NR,Brzustowicz LM,Paradiso KG,Firestein BL

PA1-9533 was used in Immunocytochemistry to demonstrate how an in vitro model of human neuronal development can help in understanding the etiology of schizophrenia and can also be used as a platform to screen drugs for patients.

Tue Dec 01 00:00:00 EST 2020

Sub-Ciliary Segregation of Two Drosophila Transient Receptor Potential Channels Begins at the Initial Stage of Their Pre-Ciliary Trafficking.

Molecules and cells

Kwon Y,Lee J,Chung YD

PA1-9533 was used in Immunohistochemistry to suggest that subciliary segregation of the two TRP channels relies on their distinct intrinsic properties, and begins at the initial stage of their pre-ciliary trafficking.

Thu Dec 31 00:00:00 EST 2020

Sub-Ciliary Segregation of Two Drosophila Transient Receptor Potential Channels Begins at the Initial Stage of Their Pre-Ciliary Trafficking.

Molecules and cells

Kwon Y,Lee J,Chung YD

Thu Dec 31 00:00:00 EST 2020

BioID-based proteomic analysis of the Bid interactome identifies novel proteins involved in cell-cycle-dependent apoptotic priming.

Cell death & disease

Pedley R,King LE,Mallikarjun V,Wang P,Swift J,Brennan K,Gilmore AP

PA1-9533 was used in Western Blot, Immunocytochemistry to identify regulators of apoptotic priming in mitosis, using Bid as bait.

Fri Oct 16 00:00:00 EDT 2020

BioID-based proteomic analysis of the Bid interactome identifies novel proteins involved in cell-cycle-dependent apoptotic priming.

Cell death & disease

Pedley R,King LE,Mallikarjun V,Wang P,Swift J,Brennan K,Gilmore AP

PA1-9533 was used in Western Blot, Immunocytochemistry to identify regulators of apoptotic priming in mitosis, using Bid as bait.

Fri Oct 16 00:00:00 EDT 2020

Encoding and control of orientation to airflow by a set of Drosophila fan-shaped body neurons.

eLife

Currier TA,Matheson AM,Nagel KI

PA1-9533 was used in Immunohistochemistry to identify a group of CX neurons that robustly encode airflow direction and are required for proper orientation to this stimulus.

Wed Dec 30 00:00:00 EST 2020

Encoding of Wind Direction by Central Neurons in Drosophila.

Neuron

Suver MP,Matheson AMM,Sarkar S,Damiata M,Schoppik D,Nagel KI

PA1-9533 was used in Immunohistochemistry to establish how a feature of the sensory environment-wind direction-is decoded by neurons that compare information across two sensors.

Wed May 22 00:00:00 EDT 2019

RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.

PLoS genetics

Pavlova GA,Popova JV,Andreyeva EN,Yarinich LA,Lebedev MO,Razuvaeva AV,Dubatolova TD,Oshchepkova AL,Pellacani C,Somma MP,Pindyurin AV,Gatti M

PA1-9533 was used in Immunocytochemistry-immunoflourescence to suggest that the mitotic phenotypes caused by Rcd1, Rcd5, MBD-R2 or Wds depletion are primarily due to reduced transcription of genes involved in kinetochore assembly and centriole duplication.

Sun Sep 01 00:00:00 EDT 2019

The role of Patronin in Drosophila mitosis.

BMC molecular and cell biology

Pavlova GA,Razuvaeva AV,Popova JV,Andreyeva EN,Yarinich LA,Lebedev MO,Pellacani C,Bonaccorsi S,Somma MP,Gatti M,Pindyurin AV

PA1-9533 was used in Immunocytochemistry-immunoflourescence to explore the role of Patronin in Drosophila mitosis using S2 tissue culture cells as a model system.

Wed Apr 17 00:00:00 EDT 2019

Inactivation of hepatic ATRX in Atrx Foxg1cre mice prevents reversal of aging-like phenotypes by thyroxine.

Aging

Rowland ME,Jiang Y,Beier F,Bérubé NG

Published figure using GFP polyclonal antibody (Product # PA1-9533) in Immunofluorescence

Thu Jun 07 00:00:00 EDT 2018

The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor.

Frontiers in cellular neuroscience

O'Neill KM,Donohue KE,Omelchenko A,Firestein BL

PA1-9533 was used in Immunocytochemistry-immunoflourescence to emphasize the importance of intracellular transport of BDNF mRNAs in the regulation of dendrite morphology.

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

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